The ratio of immune reactions in female employees of cellulose production plant

Arctic Biomonitoring

Gechavez, N.P.^1 ; Morosova, O.S.^2 ; Nekrasova, M.V.^2

Higher School of Life Sciences and Technology, M.V. Lomonosov NArFU, 4 Lomonosov Ave., Arkhangelsk, Russia^1

Federal Agency of Research Organizations (FANO-Russia), Federal State-Financed Research Institution, Russian Academy of Sciences', Academician Laverov Research Center for Integrated Study of the Arctic, 249 Lomonosov Ave., Arkhangelsk, Russia^2

关键词: Arkhangelsk pulp and paper mills;    Cellulose production;    Comprehensive analysis;    Cytotoxic activities;    Laboratory datum;    Peripheral blood;    Phagocytic activity;    Russian Academy of Sciences;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/263/1/012029/pdf
DOI  :  10.1088/1755-1315/263/1/012029

来源: IOP

【 摘 要 】

The study covered 17 women, considered at the time of examination to be healthy, aged between 20 and 39, employed by JSC "Arkhangelsk Pulp and Paper Mill" (2012). It involved the analysis of clinical and laboratory data. The series of immunological tests were conducted in the laboratory of physiology of immunocompetent cells at the Russian Academy of Sciences' Academician Laverov Research Center for Integrated Study of the Arctic (Arkhangelsk). The ulnar vein blood was taken in the amount of 6 ml. at 9-10 o'clock in the morning, on empty stomach. Blood sampling made use of IMPROVACUTER lithium-heparin vacutainers. Our comprehensive analysis of the immune status targeted to determine the content in the peripheral blood of lymphocyte phenotypes CD5+, CD8+, CD16+, CD20+, CD71+, CD95+; phagocytic activity; and phagocytic number. The content of lymphocyte phenotypes was determined through indirect immunoperoxidase reaction using monoclonal antibodies (NPC "Medbiospectr", Russia) on preparations of lymphocytes of the "dried drop" type. The percentage of active phagocytes and the phagocytic number were calculated in blood smears according to Romanovsky-Gimza. In processing the data, we made use of Statistica 6.0 software for mathematical statistics. The surveyed group of female employees of JSC "Arkhangelsk Pulp and Paper Mill" appear to have deficit of CD5+ T-lymphocytes (in 100% of the surveyed female employees) and increased cytotoxic activity (CD8+) (in 11,76% of the surveyed female employees).