Spermatozoa separation method with different percoll composition on motility, abnormalities, and mortality of local goat sperm

International Conference on Agriculture, Environment, and Food Security 2018

农业科学;生态环境科学

Patriani, P.^1 ; Hasnudi^1 ; Mirwandhono, E.^1 ; Ginting, Nurzainah^1 ; Hasanah, U.^1

Departement of Animal Science, Universitas Sumatera, Utara Medan, Indonesia^1

关键词: Artificial insemination;    Block designs;    Percoll;    Semen collection;    Separation methods;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/260/1/012059/pdf
DOI  :  10.1088/1755-1315/260/1/012059

来源: IOP

【 摘 要 】

This study aimed to determine the effect of spermatozoa separation on local goats using different percoll compositions for abnormalities, mortality, and motility. Percoll is a tool for efficient in biochemistry. It is used for the isolation of cells, organelles, or viruses by density centrifugation. The material in this study was four local goats, aged 1.5 years. The treatment in this study consisted of: P1 = Percentage of Percoll: percentage of M-199 + aquabidestillata + Bicarbonate [10:90], P2 = [20:80], P3 = [30:70] and P4 = [40:60]. The experiment was carried out six times and the semen collection interval every two days. The design of this study uses a random block design to check motility, mortality, and abnormalities. The result of this study is the separation of goat spermatozoa using different percoll compositions affecting motility, mortality, abnormalities, but no effect on sperm abnormalities. Percoll can increase the dominance of Y sperm with a high proportion in P3 and P4 90.3 ± 5.8 and 87 ± 5.2 present, respectively. Additional percoll composition can reduce motility and increase mortality, but still good enough for Artificial Insemination [AI] implementation.