Molecular Identification and Genetic Diversity of Thalassia hemprichii Through DNA Barcoding Using Internal Transcribed Spacer gene (ITS) from Awur Bay Jepara, Indonesia

1st International Conference on Life and Applied Sciences for Sustainable Rural Development

生态环境科学

Faozi, A.N.^1 ; Harisam, T.^1 ; Pharmawati, M.^2 ; Marhaeni, B.^1

Fisheries and Marine Science Faculty, Jenderal Soedirman University, Purwokerto

53122, Indonesia^1

Biology Department, Faculty of Mathematics and Natural Sciences, Udayana University, Bali, Indonesia^2

关键词: Conventional identification;    Environmental pressures;    Genetic diversity;    Internal transcribed spacers;    Molecular identification;    Morphological identifications;    PCR amplification;    Thalassia hemprichii;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/255/1/012037/pdf
DOI  :  10.1088/1755-1315/255/1/012037

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

Thalassia hemprichii is a type of seagrass that dominates in Awur Bay of Jepara. Conventional identification has many obstacles and the environmental pressure causes seagrass stand damage and incomplete. This leads to difficulty in morphological identification. Therefore, it is necessary to do molecular identification. DNA barcode using ITS gene is one method of molecular identification using short sequences that are efficient and have a high level of accuracy. The purposes of this research were to identify Thalassia hemprichii using DNA barcode of the Internal Transcribed Spacer (ITS). The research methods includeds DNA extraction using CTAB, PCR amplification of ITS fragment, electrophoresis, DNA sequencing, and analysis with MEGA 6 and BLAST. The result of this research showed that all of the samples studied were Thalassia hemprichii. DNA barcode of ITS is a gene marker which capable of identifying seagrass species of Thalassia hemprichii.