Potential and Enzymatic Characterizations of Marine Yeast for Pufas from Balai Taman Nasional Karimunjawa

4th International Conference on Tropical and Coastal Region Eco Development

生态环境科学;经济学

Devi, Sabrina Alisha^1 ; Wulandary, Dyah Ayu^1 ; Saputra, Ega^1 ; Muchlissin, Sakti Imam^2 ; Setyati, Wilis Ari^1

Faculty. of and Marine Science, Diponegoro University., Semarang, Indonesia^1

Laboraty of Tropical Marine Biotechnology^2

关键词: Colony morphology;    Culture media;    Enzymatic methods;    Mangrove ecosystems;    Marine yeasts;    Molecular identification;    Protease enzyme;    Sampling method;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/246/1/012074/pdf
DOI  :  10.1088/1755-1315/246/1/012074

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

Potential of huge mangrove ecosystem in Indonesia, especially in Balai Taman Nasional Karimunjawa, for exploration of potential microorganism. One of them were yeast. PUFA is beneficial as a source of food for low and high-level organisms. The aim of this study is to explored the potential of yeast by enzymatic methods, differences of culture media and molecular identification in Balai Taman Nasional Karimunjawa. The research flow included isolation of yeast from several mangrove ecosystem located in Karimunjawa by purposive sampling methods. The pine pollen used to bait, identification colony morphology, Protease, amylolytic, cellulolytic, Lipase Enzymatic Test, mass culture used a variety of different media, molecular identification used 18S rDNA. The results of the studied obtained 4 isolates with the code (T2, KM1, KM2, KM3) which have the potential on the lipase and protease enzyme. Molecular identification used 18S rDNA analysis showed that all isolates were the yeast. Differences in the use of media also produce various yeast biomass.