Genetic Diversity of Pummelo Germplasms in Sichuan Basin Based on RAPD Markers

4th International Conference on Advances in Energy Resources and Environment Engineering

能源学;生态环境科学

Wang, Yan^1^2 ; Qi, Youheng^1 ; He, Wen^1 ; Chen, Qing^1 ; Wang, Xiaorong^1^2

College of Horticulture, Sichuan Agricultural University, Chengdu, Sichuan

611130, China^1

Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu, Sichuan

611130, China^2

关键词: Breeding program;    Distance coefficient;    Genetic diversity;    Genetic similarity coefficients;    Genetic variation;    Polymorphic bands;    Production area;    Shatian pummelo;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/237/3/032076/pdf
DOI  :  10.1088/1755-1315/237/3/032076

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

Sichuan Basin is one of the centers of pummelo cultivation, domestication and diversity. Also, as one of the main production areas, Sichuan Basin possessed abundant pummelo germplasm resources. In this study, genetic diversity analysis among 73 accessions of pummelo (Citrus grandis Osbeck) and rootstocks from Sichuan Basin was assessed by using RAPD markers. A total of 156 bands were amplified by 15 RAPD primers, 93.59% (146) of which 146 were polymorphic with an average of 10.4 polymorphic bands per primer. The genetic similarity coefficients of 73 germplasms varied from 0.4511 to 0.9808 at the accession level. At the group level, the maximum GS coefficient index was observed between Wendan and Guanxi Miyou & bud mutants (0.8838), and the minimum GS coefficient was shown in Shatianyou and Putaoyou (0.6852). 73 accessions of pummelo and rootstocks were clustered into five groups with UPGMA method at distance coefficient of 0.6102, consisting of Shatian pummelo varieties group, Wendan pummelo varieties group, Guanxi Miyou & bud mutants group, Putao pummelo varieties group, and rootstocks of C. junos (Pujiang Xiangcheng and Ziyang Xiangcheng) and Poncirus trifoliata. In addition, Guanxi Miyou and bud mutants were clearly separated in our RAPD analysis. This study demonstrated abundant genetic variation among Guanxi Miyou and bud mutants, which could be used core germplasms in the future pummelo breeding program.