Isolation and identification of proteolytic bacteria from pig sludge and protease activity determination

International Conference on Green Agro-industry and Bioeconomy

农业科学;工业技术（总论）;经济学

Hamdani, S.^1^2 ; Asstiyani, N.^1 ; Astriany, D.^1 ; Singgih, M.^2 ; Ibrahim, S.^2

Sekolah Tinggi Farmasi Indonesia, Bandung, Indonesia^1

School of Pharmacy, Bandung Institute of Technology, Bandung, Indonesia^2

关键词: Agar media;    Agar medium;    Bacteria isolation;    Isolation and identification;    Protease activities;    Qualitative test;    Separation process;    Staphylococcus sciuri;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/230/1/012095/pdf
DOI  :  10.1088/1755-1315/230/1/012095

来源: IOP

【 摘 要 】

Proteolytic bacteria were found in many environment, some were found in soil or mud. This research was conducted to find proteolytic bacteria from pig sludge that is place for pig wallowing. Bacteria isolation was carried out by streak plate method on nutrient agar media. Qualitative test of proteolytic bacteria used skim milk agar medium. The results of isolation obtained 10 bacterias and 2 of them have potential as proteolytic bacteria. Identification of proteolitic bacteria used the 16s rRNA method, result showed that bacterias are Bacillus pseudomycoides and Staphylococcus sciuri. Protease isolation was carried out under 4°C and under room temperature during centrifugation as separation process. Protease activity from Bacillus pseudomycoides was at 4°C as much as 0.094 U/mL and was 0.082 U/mL at room temperature, while from Staphylococcus sciuri was at 4°C as much as 0.120 U/mL and was 0.098 U/mL at room temperature.