Bacterial community composition and diversity of two different forms of an organic residue of bioenergy crop

PeerJ

article

Matheus A.P. Cipriano¹  Afnan K.A. Suleiman²  Adriana P.D. da Silveira¹  Janaína B. do Carmo³  Eiko E. Kuramae² 

[1]Instituto Agronômico de Campinas

[2]Department of Microbial Ecology, Netherlands Institute of Ecology

[3]Universidade Federal de São Carlos

关键词: Bacteria;    16S rRNA;    Sugarcane;    Vinasse;    Diversity;    Bacteria isolation;    Biochemical characterization;    Nitrogen cycle genes;   

DOI  :  10.7717/peerj.6768

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

The use of residue of sugarcane ethanol industry named vinasse in fertirrigation is an established and widespread practice in Brazil. Both non-concentrated vinasse (NCV) and concentrated vinasse (CV) are used in fertirrigation, particularly to replace the potassium fertilizer. Although studies on the chemical and organic composition of vinasse and their impact on nitrous oxide emissions when applied in soil have been carried out, no studies have evaluated the microbial community composition and diversity in different forms of vinasse. We assessed the bacterial community composition of NCV and CV by non-culturable and culturable approaches. The non-culturable bacterial community was assessed by next generation sequencing of the 16S rRNA gene and culturable community by isolation of bacterial strains and molecular and biochemical characterization. Additionally, we assessed in the bacterial strains the presence of genes of nitrogen cycle nitrification and denitrification pathways. The microbial community based on 16S rRNA sequences of NCV was overrepresented by Bacilli and Negativicutes while CV was mainly represented by Bacilli class. The isolated strains from the two types of vinasse belong to class Bacilli, similar to Lysinibacillus, encode for nirK gene related to denitrification pathway. This study highlights the bacterial microbial composition particularly in CV what residue is currently recycled and recommended as a sustainable practice in sugarcane cultivation in the tropics.

【 授权许可】

CC BY