Effect of Cr(VI) and bisphenol A on the formation of 8-hydroxy-2' deoxyguanosine DNA adduct from 2' -deoxyguanosine through a Fenton-like reaction

2nd International Conference on Current Progress in Functional Materials 2017

Budiawan^1 ; Kusumowardani, E.^1 ; Handayani, S.^1 ; Dani, I.C.^1 ; Bakri, R.^1

Department of Chemistry, Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Indonesia, Depok

16424, Indonesia^1

关键词: 8-OHdG;    Concentration values;    Deoxyguanosine (dG);    DNA adducts;    Fenton-like reactions;    Limit of detection;    Limit of quantifications;    Reversed phase high performance liquid chromatography (HPLC);   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/496/1/012046/pdf
DOI  :  10.1088/1757-899X/496/1/012046

来源: IOP

【 摘 要 】

In this research, we study the effect of the addition of bisphenol A (BPA) and Cr(VI) on the formation of the DNA adduct, 8-hydroxy-2'-deoxyguanosine (8-OHdG). The formation of 8-OHdG, which is monitored using reversed phase high performance liquid chromatography (HPLC) with a Ultraviolet-Visible (UV-Vis) detector at 254 nm, can be achieved through the reaction of 2' -deoxyguanosine (dG) with BPA in the presence of Fenton-like reagents and vitamin C. The reaction is performed under different conditions, including several pH (7.4 and 8.4), temperatures (37 °C and 60 °C), and incubation times (7 and 12 h). To detect 8-OHdG, the minimum concentration was set as the limit of detection (LOD) value of 5.19 ppb, and the minimum concentration for its quantification was set as the limit of quantification (LOQ) value of 17.29 ppb. The results of this study indicate that the concentration of 8-OHdG increases upon addition of BPA and Cr(VI), and most of the concentration values obtained from the analysis are greater than the LOD value. The addition of Fenton-like reagent and vitamin C causes an increase in the concentration of 8-OHdG. For most samples, the conditions that cause the highest 8-OHdG concentration are a pH of 7.4, a temperature of 60 °C, and an incubation time of 12 h.