Performance of SGPN-NPs paste electrode as medical biosensor

International Conference on Mechanical Engineering Research and Application

Rohmah, Faizatur^1 ; Kurniawan, Fredy^2

Department of Heavy Equipment Engineering, State Polytechnic of Madura, Sampang, East Java, Indonesia^1

Laboratory of Instrumentation and Analytical Sciences, Chemistry Department, Faculty of Sciences, Institut Teknologi Sepuluh Nopember, Surabaya, East Java

60111, Indonesia^2

关键词: Calibration curves;    Comparison result;    Electrochemical response;    Limit of detection;    Nickel nanoparticles;    Paste electrodes;    Phosphate buffer solutions;    Silver electrode;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/494/1/012058/pdf
DOI  :  10.1088/1757-899X/494/1/012058

来源: IOP

【 摘 要 】

SGPN-NPs paste electrode as biosensor have been successfully fabricated. The electrode was made by modification material such as silica gel, solid paraffin, liquid paraffin and nickel nanoparticles. All modification material would be mixed into paste and then modified on the silver electrode which covered by glass tube. Comparison result will be studied by silver electrode with and without modification. The performance of the biosensor was investigated in medical sample, i.e. insulin. The electrochemical response from the biosensor was performed by cyclic voltammetry (CV) in phosphate buffer solution (pH = 7.0) at room temperature. The best response of the biosensor was achieved by silver electrode with modification. This SGPN-NPs paste electrode can detect insulin at potential of -0.667 V. The obtained calibration curve followed a linear equation, Ipc = -5.1350c - 330.2550, with R2 = 0.97564. The limit of detection (LOD) and sensitivity of the biosensor for insulin detection were 4.92257 nM and 1.6353 μA.nM-1.mm-2, respectively. The result confirm that SGPN-NPs paste electrode is favorable for detecting medical sample, especially insulin.