In recent years, antibiotics pollution has caused serious harm to the aquatic environment, and microalgae mediated degradation of antibiotics has attracted increasing attention. However, the potential toxicity of antibiotics to keystone microalgae species or their microalgae consortia, and the impact of microalgal diversity on antibiotic removal need to be further studied. In this study, we investigated the removal efficiency and tolerance of five freshwater microalgae (Chlorella pyrenoidosa, Scenedesmus quadricauda, Dictyosphaerium sp., Haematoccocus pluvialis, and Botryococcus braunii) and their microalgae consortia to sulfamethoxazole (SMX). We found that the removal efficiency of SMX by C. pyrenoidosa reached 49%, while the other four microalgae ranged between 9% and 16%. In addition, C. pyrenoidosa, S. quadricauda, and Dictyosphaerium sp. had better tolerance to SMX than H. pluvialis, and their growth and photosynthesis were less affected. At 10 and 50 mg/L SMX, the removal capacity of SMX by mixed microalgae consortia was lower than that of C. pyrenoidos except for the consortium with C. pyrenoidos and S. quadricauda. The consortia generally showed higher sensitivity towards SMX than the individual species, and the biochemical characteristics (photosynthetic pigment, chlorophyll fluorescence parameters, superoxide anion (O2-), superoxide dismutase activity (SOD), malondialdehyde (MDA) and extracellular enzymes) were significantly influenced by SMX stress. Therefore, the removal of antibiotics by microalgae consortia did not increase with the number of microalgae species. Our study provides a new perspective for the selection of microalgal consortia to degrade antibiotics.

Faults along the boundaries of active tectonic blocks are the main structures that are responsible for major earthquakes in mainland China. Investigating the geometric distribution, rupture behavior, and paleoseismic history of these faults is the prerequisite for constraining geodynamic models and regional seismic hazard analyses. The Nanhe Fault, located at the eastern boundary of the Sichuan–Yunnan Block near Mianning County, has been paid less attention so far due to insufficient historical records of major earthquakes. In this paper, we focused on the Nanhe Fault and conducted satellite imagery interpretation, field investigations, and trench excavations. Our findings indicate that the Nanhe Fault initiates north of Mianning County; the north segment of the fault is connected with the Anninghe Fault; and it extends for about 70 km south-westward and terminates southwest of Ermaga Village. The fault has been faulting in the late Late Pleistocene with a left-lateral strike-slip rate of 2.40–2.56 mm/yr, while in the late Holocene, the left-lateral strike-slip and vertical slip rates are 2.50–2.60 mm/yr and about 0.60 mm/yr, respectively. Three paleoseismic events (5373–4525 BC, AD 1193–1576, and AD 1496–1843) were identified by excavating trenches at the Nanhe Fault. A comparative analysis of paleoseismic events between the Nanhe and the Anninghe fault indicates that both faults may have induced cascade rupture or triggered earthquakes—such related events may have occurred in 1496–1627. Additionally, by comparing the kinematic relationship of the faults at the eastern boundary of the Sichuan–Yunnan Block, we propose that the Nanhe Fault takes part in strain partitioning along the boundary. This interpretation reasonably explains the loss of the sliding rate between the Anninghe and Zemuhe faults, which also supports the GPS inversion results, and the discontinuous deformation model for the eastern margin of the Tibetan Plateau.

An extremely heavy rainfall with dual rainbands occurred in the Fujian province on 22 May 2014, causing severe disasters in Fujian. In order to investigate the key forecasting factors and the predictability of this case, the evaluation and sensitivity analysis of precipitation forecasts by the ensemble prediction system which is based on the European Centre for Medium-Range Weather Forecasts (ECMWF) global Model are carried out. The result show that the ECMWF-EPS have better ability to capture the intensity and spatial distribution of the northern rainband, but significantly underestimated the precipitation in the warm area. Through ensemble forecast sensitivity analysis and comparison between good and poor members, the main factors causing forecast deviations in the two rainbands and affecting the predictability of heavy rainfall were revealed. The forecast of rainfall distribution and intensity in the northern rainband was highly sensitive to the predictability of the weather-scale shear line. The westward bias in the forecasted position and the weakened intensity of the shear line were the main causes of the westward and weaker forecast of heavy precipitation in the northern region. Additionally, the forecast of 850 hPa low-level jets, especially the forecasted intensity of zonal winds, which were closely related to the shear line, significantly influenced the intensity forecast of precipitation in the northern region. The forecast of precipitation in the southern warm area, which was far from the shear line, was more sensitive to the wind speed and thermodynamic conditions of the southwestern airflow in the boundary layer of the upstream inflow region. Most ensemble forecast members underestimated the intensity of the southwestern airflow in the coastal boundary layer of South China, which was the main reason for the near omission of heavy rainfall in the southern warm area. This study provides a quantitative correlation between low-level southwest jets along the coast of Guangdong and Fujian and non-typhoon heavy rainfall in Fujian, and explores their impact on heavy precipitation forecasts.

Background and aimsIn agriculture, biochar (BC) and nitrogen (N) fertilizers are commonly used for improving soil fertility and crop productivity. However, it remains unclear how different levels of BC and N fertilizer affect soil fertility and crop productivity.MethodsThis study elucidates the impact of different application rates of BC (0, 600, and 1200 kg/ha) and N fertilizer (105 and 126 kg/ha) on biomass accumulation, soil microbial biomass of carbon (SMC) and nitrogen (SMN), and soil biochemical properties, including soil organic carbon (SOC), total nitrogen (TN), soil nitrate nitrogen (NO3−−N), ammonium nitrogen (NH4+−N), urease (UE), acid phosphatase (ACP), catalase (CAT), and sucrase (SC) of tobacco plants. In addition, a high throughput amplicon sequencing technique was adopted to investigate the effect of different application rates of BC/N on rhizosphere bacterial communities of tobacco plants.ResultsThe results confirm that high dosages of BC and N fertilizer (B1200N126) significantly enhance dry matter accumulation by 31.56% and 23.97% compared with control B0N105 and B0N126 under field conditions and 23.94% and 24.52% under pot experiment, respectively. The soil biochemical properties, SMC, and SMN significantly improved under the high application rate of BC and N fertilizer (B1200N126), while it negatively influenced the soil carbon/nitrogen ratio. Analysis of rhizosphere bacteriome through amplicon sequencing of 16S rRNA revealed that the structure, diversity, and composition of rhizosphere bacterial communities dramatically changed under different BC/N ratios. Proteobacteria, Bacteroidetes, Actinobacteria, Firmicutes, and Acidobacteria were highly abundant bacterial phyla in the rhizosphere of tobacco plants under different treatments. Co-occurrence network analysis displayed fewer negative correlations among rhizosphere bacterial communities under high dosages of biochar and nitrogen (B1200N126) than other treatments, which showed less competition for resources among microbes. In addition, a redundancy analysis further proved a significant positive correlation among SMC, SMN, soil biochemical properties, and high dosage of biochar and nitrogen (B1200N126).ConclusionsThus, we conclude that a high dosage of BC (1200 kg/ha) under a high application rate of N fertilizer (126 kg/ha) enhances the biomass accumulation of tobacco plants by improving the soil biochemical properties and activities of rhizosphere bacterial communities.

BackgroundTocotrienols and tocopherols, which are synthesized in plastids of plant cells with similar functionalities, comprise vitamin E to serve as a potent lipid-soluble antioxidant in plants. The synthesis of tocopherols involves the condensation of homogentisic acid (HGA) and phytyl diphosphate (PDP) under the catalysis of homogentisate phytyltransferase (HPT). Tocotrienol synthesis is initiated by the condensation of HGA and geranylgeranyl diphosphate (GGDP) mediated by homogentisate geranylgeranyl transferase (HGGT). As one of the most important oil crops, canola seed is regarded as an ideal plant to efficiently improve the production of vitamin E tocochromanols through genetic engineering approaches. However, only a modest increase in tocopherol content has been achieved in canola seed to date.MethodsIn this study, we transformed barley HGGT (HvHGGT) into canola to improve total tocochromanol content in canola seeds.Results and discussionThe results showed that the total tocochromanol content in the transgenic canola seeds could be maximally increased by fourfold relative to that in wild-type canola seeds. Notably, no negative impact on important agronomic traits was observed in transgenic canola plants, indicating great application potential of the HvHGGT gene in enhancing tocochromanol content in canola in the future. Moreover, the oil extracted from the transgenic canola seeds exhibited significantly enhanced oxidative stability under high temperature in addition to the increase in total tocochromanol content, demonstrating multiple desirable properties of HvHGGT.