A novel turn-on fluorescence assay was developed for the rapid detection of glutathione (GSH) based on the inner-filter effect (IFE) and redox reaction. Molybdenum disulfide quantum dots (MoS2 QDs), which have stable fluorescent properties, were synthesized with hydrothermal method. Manganese dioxide nanosheets (MnO2 NSs) were prepared by exfoliating the bulk δ-MnO2 material in bovine serum albumin (BSA) aqueous solution. The morphology structures of the prepared nanoparticles were characterized by transmission electron microscope (TEM). Studies have shown that the fluorescence of MoS2 QDs could be quenched in the presence of MnO2 NSs as a result of the IFE, and is recovered after the addition of GSH to dissolve the MnO2 NSs. The fluorescence intensity showed a good linear relationship with the GSH concentration in the range 20–2500 μM, the limit of detection was 1.0 μM. The detection method was applied to the analysis of GSH in human serum samples. This simple, rapid, and cost-effective method has great potential in analyzing GSH and in disease diagnosis.

Background: Ambiguities and anomalies in the Activity of Daily Living (ADL) patterns indicate deviations from Wellness. The monitoring of lifestyles could facilitate remote physicians or caregivers to give insight into symptoms of the disease and provide health improvement advice to residents; Objective: This research work aims to apply lifestyle monitoring in an ambient assisted living (AAL) system by diagnosing conduct and distinguishing variation from the norm with the slightest conceivable fake alert. In pursuing this aim, the main objective is to fill the knowledge gap of two contextual observations (i.e., day and time) in the frequent behavior modeling for an individual in AAL. Each sensing category has its advantages and restrictions. Only a single type of sensing unit may not manage composite states in practice and lose the activity of daily living. To boost the efficiency of the system, we offer an exceptional sensor data fusion technique through different sensing modalities; Methods: As behaviors may also change according to other contextual observations, including seasonal, weather (or temperature), and social interaction, we propose the design of a novel activity learning model by adding behavioral observations, which we name as the Wellness indices analysis model; Results: The ground-truth data are collected from four elderly houses, including daily activities, with a sample size of three hundred days plus sensor activation. The investigation results validate the success of our method. The new feature set from sensor data fusion enhances the system accuracy to (98.17% ± 0.95) from (80.81% ± 0.68). The performance evaluation parameters of the proposed model for ADL recognition are recorded for the 14 selected activities. These parameters are Sensitivity (0.9852), Specificity (0.9988), Accuracy (0.9974), F1 score (0.9851), False Negative Rate (0.0130).