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  • × Ying Wang
  • × 期刊论文
  • × Scientific Reports
  • × 2017
 全选  【符合条件的数据共：7条】

1 The ideal harvest time for seeds of hybrid maize (Zea mays L.) XY335 and ZD958 produced in multiple environments [期刊论文]

Scientific Reports,2017年

Li Li, Jianhua Wang, Riliang Gu, Tinglu Fan, Ying Wang, Xiaolin Liang, Yanbo Wang

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2 Nano-silver-decorated microfibrous eggshell membrane: processing, cytotoxicity assessment and optimization, antibacterial activity and wound healing [期刊论文]

Scientific Reports,2017年

Jianglin Tan, Jun Wu, Menglong Liu, Rui Xu, Weifeng He, Ying Wang, Gaoxing Luo, Malcolm Xing, Yuzhen Wang

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3 A tricyclic antidepressant, amoxapine, reduces amyloid-β generation through multiple serotonin receptor 6-mediated targets [期刊论文]

Scientific Reports,2017年

Qinying Wang, Xiaohang Li, Gang Pei, Tingting Hu, Jing Lu, Ying Wang, Jian Zhao

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4 Protective Efficacy of the Trivalent Pseudomonas aeruginosa Vaccine Candidate PcrV-OprI-Hcp1 in Murine Pneumonia and Burn Models [期刊论文]

Scientific Reports,2017年

Feng Yang, Fenglin Lv, Quanming Zou, Haiming Jing, Liuyang Yang, Hao Zeng, Jiang Gu, Ying Wang, Chen Gao, Jinyong Zhang

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5 Evolution of the Araliaceae family inferred from complete chloroplast genomes and 45S nrDNAs of 10 Panax-related species [期刊论文]

Scientific Reports,2017年

Sang-Choon Lee, Kyunghee Kim, Jee Young Park, Van Binh Nguyen, Tae-Jin Yang, Ying Wang, Jingzhou Dong

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6 Generation of complement protein C3 deficient pigs by CRISPR/Cas9-mediated gene targeting [期刊论文]

Scientific Reports,2017年

Wei Zhang, Yong Jin, Lihua Zhao, Lining Zhang, Rongfeng Li, Guan Wang, Qiang Xiong, Haiyuan Yang, Ying Wang, Yifan Dai, Lisha Mou

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Abstract Complement protein C3 is the pivotal component of the complement system. Previous studies have demonstrated that C3 has implications in various human diseases and exerts profound functions under certain conditions. However, the delineation of pathological and physiological roles of C3 has been hampered by the insufficiency of suitable animal models. In the present study, we applied the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) system to target the C3 gene in porcine fetal fibroblasts. Our results indicated that CRISPR/Cas9 targeting efficiency was as high as 84.7%, and the biallelic mutation efficiency reached at 45.7%. The biallelic modified colonies were used as donor for somatic cell nuclear transfer (SCNT) technology to generate C3 targeted piglets. A total of 19 C3 knockout (KO) piglets were produced and their plasma C3 protein was undetectable by western blot analysis and ELISA. The hemolytic complement activity and complement-dependent cytotoxicity assay further confirmed that C3 was disrupted in these piglets. These C3 KO pigs could be utilized as a valuable large animal model for the elucidation of the roles of C3.