Cell Discovery,2020年
Hao Li, Wei Liu, Haolong Zeng, Hongbo Chen, Runming Jin, Lei-Ke Zhang, Qingxing Wang, Gengfu Xiao, Yan Wu, Shufen Li, Xiaming Jiang, Yuan Sun, Yu-Lan Zhang, Ke Peng, Wei-Juan Shang, Zhao-Nian Hao
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] Cell Discovery,2020年
Yulan Zhang, Xiulian Sun, Di Liu, Ke Peng, Wuxiang Guan, Zhiwei Sui, Qing-Bin Lu, Min Huang, Han Zhao, Liqun Fang, Wei Liu
LicenseType:CC BY |
Cell Discovery,2022年
Min Liu, Zhangqi Xu, Chunxiao Huo, Xiying Chen, Yongxia Chang, Tianhua Zhou, Yuehong Yang, Xiaoyi Yan, Wei Liu, Jianzhao Liu, Ting Li, Feng Yang, Shanshan Xie, Qiang Shu, Wenjun Kuang, Hao Jin
LicenseType:Unknown |
Cell Discovery,2020年
Hao Li, Wei Liu, Haolong Zeng, Runming Jin, Hongbo Chen, Ke Peng, Qingxing Wang, Lei-Ke Zhang, Gengfu Xiao, Yuan Sun, Wei-Juan Shang, Yu-Lan Zhang, Xiaming Jiang, Shufen Li, Yan Wu, Zhao-Nian Hao
LicenseType:Unknown |
Cell Discovery,2023年
Wasim Shah, Baolu Shi, Yuanwei Zhang, Qinghua Shi, Yuewen Wang, Xiaohua Jiang, Yang Li, Zishuo Xu, Wei Liu, Xuefeng Xie, Xiangjun Zhang, Muhammad Zubair, Hui Ma, Yue Wang, Huan Zhang, Bo Xu, Jianteng Zhou, Suixing Fan, Yuying Jiao, Jingwei Ye, Hanwei Jiang, Zhipeng Xu
LicenseType:CC BY |
During meiosis, at least one crossover must occur per homologous chromosome pair to ensure normal progression of meiotic division and accurate chromosome segregation. However, the mechanism of crossover formation is not fully understood. Here, we report a novel recombination protein, C12ORF40/REDIC1, essential for meiotic crossover formation in mammals. A homozygous frameshift mutation in C12orf40 (c.232_233insTT, p.Met78Ilefs*2) was identified in two infertile men with meiotic arrest. Spread mouse spermatocyte fluorescence immunostaining showed that REDIC1 forms discrete foci between the paired regions of homologous chromosomes depending on strand invasion and colocalizes with MSH4 and later with MLH1 at the crossover sites. Redic1 knock-in (KI) mice homozygous for mutation c.232_233insTT are infertile in both sexes due to insufficient crossovers and consequent meiotic arrest, which is also observed in our patients. The foci of MSH4 and TEX11, markers of recombination intermediates, are significantly reduced numerically in the spermatocytes of Redic1 KI mice. More importantly, our biochemical results show that the N-terminus of REDIC1 binds branched DNAs present in recombination intermediates, while the identified mutation impairs this interaction. Thus, our findings reveal a crucial role for C12ORF40/REDIC1 in meiotic crossover formation by stabilizing the recombination intermediates, providing prospective molecular targets for the clinical diagnosis and therapy of infertility.
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