- 已选条件：
-
× Mourad Assidi
Tumor Biology,2019年
Mohammed Al-Qahtani, Abdelbaset Buhmeida, Asia Al-Harbi, Mahmoud Al-Ahwal, Jaudah Al-Maghrabi, Mehenaz Hanbazazh, Wafaey Gomaa, Mourad Assidi, Mohammad Jafri, Peter Pushparaj
LicenseType:Unknown |
| 
| 
[ 浏览：0 
下载：0
] Libyan Journal of Medicine,2021年
Mourad Assidi, Heba Alkhatabi, Mohammad Alam Jafri, Muhammad Abu-Elmagd, Abdelbaset Buhmeida, Peter N. Pushparaj, Abdelfatteh El Omri, Jaudah Al-Maghrabi, Salina Saddick, Hussain Basalamah, Hesham Sait, Khalid Sait, Nisreen Anfinan, Maram Sait, Safia Messaoudi
LicenseType:Unknown |
BMC Genomics,2017年
Mourad Assidi, Abdelbaset Buhmeida, Mohammed Al-Qahtani, Muhammad Abu-Elmagd, Jennifer D. Churchill, Bruce Budowle, Angie D. Ambers, Jonathan L. King, Monika Stoljarova, Harrell Gill-King
LicenseType:Unknown |
BMC Genomics,2016年
Mourad Assidi, Mohammed H. Al Qahtani, Muhammad Abu-Elmagd, Faten Hachani Ben Ali, Sondes Hizem, Fatma Megdich, Faouzi Janhai, Assila Ben Salem, Malek Souayeh, Touhami Mahjoub, Mounir Ajina, Olfa Kacem
LicenseType:Unknown |
BMC Genomics,2016年
Mourad Assidi, Adel M. Abuzenadah, Mohammed Al-Qahtani, Osama S. Bajouh, Samera F. AlBasri, Rola F. Turki, Iftikhar A. Tayubi, Mohd Rehan, Ejaz Ahmad, Mohammad S. Jamal, Ishfaq A. Sheikh, Ghazi A. Damanhouri, Mohd A. Beg
LicenseType:Unknown |
BMC Cancer,2015年
Mohammed H Alqahtani, Mourad Assidi, Muhammad Abu-Elmagd, Elie Barbour, Taha Kumosani, Ahmed Al-Hejin, Aleksandra Niedzwiecki, Mathias Rath, Rania Azar, Mona Diab-Assaf, Ghazi A Damanhouri, Steve Harakeh, Esam Azhar
LicenseType:CC BY |
BackgroundAdult T-cell Leukemia (ATL) is a disease with no known cure. The disease manifests itself as an aggressive proliferation of CD4+ cells with the human T-cell Lymphotropic virus type 1 (HTLV-1). The leukemogenesis of the virus is mainly attributed to the viral oncoprotein. Tax activates the Nuclear Factor kappa B (NF-κB) which stimulates the activity and expression of the matrix metalloproteinase-9 (MMP-9). The objective of this study was to investigate the efficacy of a specific nutrient synergy (SNS) on proliferation, Tax expression, NF-κB levels as well as on MMP-9 activity and expression both at the transcriptional and translational levels in two HTLV-1 positive cell lines, HuT-102 and C91-PL at 48h and 96h of incubation. Cytotoxicity of Epigallocatechin-3-gallate (EGCG) was assayed using CytoTox 96 Non-radioactive and proliferation was measured using Cell Titer96TM Nonradioactive Cell Proliferation kit (MTT- based assay). Enzyme linked immunosorbant assay (ELISA) and electrophoretic mobility shift assay (EMSA) were used to assess the effect of SNS on NF-κB mobility. Zymography was used to determine the effects of SNS on the activity and secretion of MMP-9. The expression of MMP-9 was done using RT-PCR at the translational level and Immunoblotting at the transcriptional level.ResultsA significant inhibition of proliferation was seen in both cell lines starting at a concentration of 200μg/ml and in a dose dependent manner. SNS induced a dose dependent decrease in Tax expression, which was paralleled by a down-regulation of the nuclearization of NF-κB. This culminated in the inhibition of the activity of MMP-9 and their expression both at the transcriptional and translational levels.ConclusionsThe results of this study indicate that a specific nutrient synergy targeted multiple levels pertinent to the progression of ATL. Its activity was mediated through the NF-κB pathway, and hence has the potential to be integrated in the treatment of this disease as a natural potent anticancer agent.
878987797
028-85220240
