Molecules,2009年
Rui-Juan Xing, Jian Wang, Li Pan
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] Molecules,2009年
Sheng Chang, Shi-Liang Yin, Jian Wang, Yong-Kui Jing
LicenseType:CC BY |
BMC Cancer,2009年
Per Ø Enger, Rolf Bjerkvig, Martha Chekenya, Sverre Mørk, Peng Zhao, Xingang Li, Narve Brekkå, Hege Jacobsen, Peter C Huszthy, Per Ø Sakariassen, Hrvoje Miletic, Jian Wang
英文
Background
Establishing clinically relevant animal models of glioblastoma multiforme (GBM) remains a challenge, and many commonly used cell line-based models do not recapitulate the invasive growth patterns of patient GBMs. Previously, we have reported the formation of highly invasive tumour xenografts in nude rats from human GBMs. However, implementing tumour models based on primary tissue requires that these models can be sufficiently standardised with consistently high take rates.
Methods
In this work, we collected data on growth kinetics from a material of 29 biopsies xenografted in nude rats, and characterised this model with an emphasis on neuropathological and radiological features.
Results
The tumour take rate for xenografted GBM biopsies were 96% and remained close to 100% at subsequent passages in vivo, whereas only one of four lower grade tumours engrafted. Average time from transplantation to the onset of symptoms was 125 days ± 11.5 SEM. Histologically, the primary xenografts recapitulated the invasive features of the parent tumours while endothelial cell proliferations and necrosis were mostly absent. After 4-5 in vivo passages, the tumours became more vascular with necrotic areas, but also appeared more circumscribed. MRI typically revealed changes related to tumour growth, several months prior to the onset of symptoms.
Conclusions
In vivo passaging of patient GBM biopsies produced tumours representative of the patient tumours, with high take rates and a reproducible disease course. The model provides combinations of angiogenic and invasive phenotypes and represents a good alternative to in vitro propagated cell lines for dissecting mechanisms of brain tumour progression.
BMC Cancer,2009年
Per Ø Enger, Rolf Bjerkvig, Martha Chekenya, Sverre Mørk, Peng Zhao, Xingang Li, Narve Brekkå, Hege Jacobsen, Peter C Huszthy, Per Ø Sakariassen, Hrvoje Miletic, Jian Wang
英文
Background
Establishing clinically relevant animal models of glioblastoma multiforme (GBM) remains a challenge, and many commonly used cell line-based models do not recapitulate the invasive growth patterns of patient GBMs. Previously, we have reported the formation of highly invasive tumour xenografts in nude rats from human GBMs. However, implementing tumour models based on primary tissue requires that these models can be sufficiently standardised with consistently high take rates.
Methods
In this work, we collected data on growth kinetics from a material of 29 biopsies xenografted in nude rats, and characterised this model with an emphasis on neuropathological and radiological features.
Results
The tumour take rate for xenografted GBM biopsies were 96% and remained close to 100% at subsequent passages in vivo, whereas only one of four lower grade tumours engrafted. Average time from transplantation to the onset of symptoms was 125 days ± 11.5 SEM. Histologically, the primary xenografts recapitulated the invasive features of the parent tumours while endothelial cell proliferations and necrosis were mostly absent. After 4-5 in vivo passages, the tumours became more vascular with necrotic areas, but also appeared more circumscribed. MRI typically revealed changes related to tumour growth, several months prior to the onset of symptoms.
Conclusions
In vivo passaging of patient GBM biopsies produced tumours representative of the patient tumours, with high take rates and a reproducible disease course. The model provides combinations of angiogenic and invasive phenotypes and represents a good alternative to in vitro propagated cell lines for dissecting mechanisms of brain tumour progression.
猪基因组注释及全基因组基因芯片设计,2009年
Ruiqiang Li, Yingrui Li, Xiaodong Fang, Huanming Yang, Jian Wang, Karsten Kristiansen, Jun Wang
LicenseType:Others | 英文
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