Chromatographic and chemical analysis of Sarcostemma viminale R. Br. Research Article First Online: 15 July 2014 Received: 28 October 2013 Accepted: 24 April 2014 DOI :
10.1007/s13596-014-0157-3
Cite this article as: Girme, A., Bhalke, R., Nirmal, S. et al. Orient Pharm Exp Med (2014) 14: 279. doi:10.1007/s13596-014-0157-3 Abstract Sarcostemma viminale R. Br. (family-asclepiadaceae) is known as the “soma ” plant in India. It is rich in fatty acids and hydrocarbons and used traditionally with different medicinal use. In the present study first time systemic phytochemical profile of fatty acid was established for S. Viminale. The major fatty acids isolated and identified were1-Hexadecene (C16 H32 ), Hexadecanoic acid (C16 H32 O2 ), Octadecanoic acid (C18 H36 O2 ), 9-Octadecenoic acid (C18 H34 O2 ), 1- Docosene (C22 H44 ). Also eight major compounds were isolated and identified as Eicosane, Docosane, 1-Octadecene, Nonacosane, α- amyrin, β- amyrin, β-Sitosterol and Gallic acid. The structure elucidation was carried out using combination of Gas Chromatographic- Mass Spectroscophy (GC-MS), Fourier transform infrared spectroscopy (FT-IR), and Carbon Nuclear magnetic resonance (13 C NMR) data. This investigation is also supported with standardized phytochemical studies and moderate anti-bacterial activity of the plant.
Keywords Sarcostemma viminale Phytochemical investigation Fatty acid GC-MS Anti-bacterial activity An erratum to this article can be found at http://dx.doi.org/10.1007/s13596-014-0164-4
References Beckett AK, Stenlake JB (2001) Practical Pharmaceutical Chemistry. CBS Publisher and Distributors, New Delhi, p 86
Google Scholar Brain KR, Turner TD (1975) The Practical Evaluation of Phytopharmaceuticals. John Wright and Sons, London, p 86
Google Scholar Ferreira BS, Faza LP, Le Hyaric MA (2012) Comparison of the physicochemical properties and fatty acid composition of indaiá (
Attalea dubia ) and Babassu (
Orbignya phalerata ) oils. Sci World J 2012:532374. doi:
10.1100/2012/532374 Google Scholar Girme AS, Nirmal SA, Bhalke RD, Chavan MJ (2008) Analgesic, CNS Depressant and Anthelmintic activity of
Sarcostemma viminale . Iranian Journal of Pharmacology & Therapeutics 7(2):153–156
Google Scholar Janssen AM, Scheffer JJC, Svendsen A (1987) Antimicrobial activity of essential oils: a literature review. Aspects of the test methods Planta Med 53(5):395–8
Google Scholar Jirovetz L, Buchbauer G, Shafi MP, Leela NK (2003) Analysis of the essential oils of the leaves, stems, rhizomes and roots of the medicinal plant
Alpinia galanga from southern India. Acta Pharm 53:73–81
PubMed Google Scholar Khandelwal KR. (2000) Practical Pharmacognosy, Nirali Prakashan. pp-10, 27–37, 47–50
Khare NK, Khare MP, Khare A, Kumar R (1987) A novel pregnane derivative from
Sarcostemma brevistigma . J Nat Prod 50(1):600–603
PubMed CrossRef Google Scholar Kirtika KR, Basu BD (1984) Indian Medicinal Plant. ShivaPublishers, Dehradun, pp 1621–1624
Google Scholar Kumar PS, Soni K, Saraf MN (2006) In vitro tocolytic activity of
Sarcostemma brevistigma Wight. Ind J Pharm Sci 68(190–200):194
Google Scholar Lalitha KG, Sethuraman MG, Rajkapoor B (2002) Anti-inflammatory Activity of
Sarcostemma brevistigma in rats. Ind Jr Pharm Sci 65(2):210–212
Google Scholar Lorenzo D, Paz D, Davies P, Villamil J, Vila R, Cañigueral S, Dellacassa E (2005) Application of multidimensional gas chromatography to the enantioselective characterisation of the essential oil of
Eupatorium buniifolium Hooker et Arnott. Phytochem Anal 16(1):39–44
PubMed CrossRef Google Scholar Mukherjee PK (2002) Quality control of herbal drugs
, Business Horizones. Pharmaceutical Publishers, New Delhi, pp 405–407
Google Scholar Nirmal SA, Girme AS, Bhalke RD (2007) Major constituents and anthelmintic activity of volatile oils from leaves and flowers of
Cymbopogon martini Roxb. Nat Prod Res 21(13):1217–1220
PubMed CrossRef Google Scholar Nogueira JMF, Romano A (2002) Essential oils from micropropagated plants of
Lavandula viridis Phytochem. Anal 13:4–7
Google Scholar Oberoi K, Khare MP, Khare A (1985) A pregnane ester triglycoside from. Sarcostemma brevistigma Phytochemistry 24(12):3011–3014
Pelczar, MJ, Chan, ECS. (1993) Noel-Kreig, Microbiology, M. C. Grow Hill Inc. New York, pp-106
Pharmacopoeia of India, (1985). Controller of Publication, New Delhi. pp -A-124, A-74, A-73
Saraf MN, Patwardhan BK (1988) Pharmacological studies on
Sarcostemma brevistigma W. part. Ind. Drugs 26(2):49–53
Google Scholar Schaub F, Stocklin W, Reichstein T (1968) The structure of Genin G (12-O-benzoyl-desacetylmetaplexigenin) and H (di-O-benzoyl-viminolon) from the stems of
Sarcostemma viminale (L.) R. Br. Helv Chim Acta 51:767–772
PubMed CrossRef Google Scholar Sethuraman MG, Lalitha KG, Rajkapoor B (2003) Hepatoprotective activity of
Sarcostemma brevistigma against carbon tetrachloride-induced hepatic damage in rats. Curr Sci 84(9):1186–1187
Google Scholar Singh NP, Lakshminarasimhan P, Karthikeyan S,Prasanna, PV (2001) Flora of India; Series 2,Botanical survey of India, pp-1080
The wealth of India (2004) Council of Scientific & Research, New Delhi, 5 (57)
Thomson EB (1990) Drug Bio-Screening. V. C. H. Publication, New York, pp 53–78
Google Scholar Torrance JD, Marais JL (1963) Triterpene constituents of
Sarcostemma viminale R.Br. J Pharm Sci 52:439–441
PubMed CrossRef Google Scholar Verma PK, Sharma A, Mathur P, Gupta RS, Joshi SC, Dixit VP (2002) Effect of
Sarcostemma acidum stem extract on spermatogenesis in male albino rats. Asian J Androl 4(1):43–47
Google Scholar Vleggaar R, Van Heerden FR, Anderson LAP, Erasmus GL (1993) Toxic constituents of the Asclepiadaceae. Structure elucidation191 of sarcovimiside A-C, pregnane glycosides of. Sarcostemma viminale J Chem Soc 4:483–487
Google Scholar Wagnar H, Bladt S, (1996) Plant Drug Analysis; Springer. 2: pp-335, 195
© Institute of Korean Medicine, Kyung Hee University 2014
Authors and Affiliations 1. Department of Pharmacognosy Pravara Rural College of Pharmacy Pravaranagar India 2. Department of Pharmacognosy Sanjivani College of Pharmaceutical Education and Research Kopargaon India 3. Department of Pharmacognosy Amrutvahini College of Pharmacy Sangamner India 4. Interdisciplinary School of Health Sciences University of Pune Pune India