Boswellic acid suppresses cyclophosphamide induced chromosomal damage in mice

Kuntal Ganguly; N. V. Jagadeesh; Rakesh Singh; B. S. Thippeswami; Ashok D. Taranalli; Anandrao R. Kulkarni

doi:10.1007/s13596-011-0023-5

Oriental Pharmacy and Experimental Medicine

September 2011, Volume 11, Issue 3, pp 177–181

Boswellic acid suppresses cyclophosphamide induced chromosomal damage in mice

Authors
Authors and affiliations

Kuntal Ganguly
N. V. Jagadeesh
Rakesh Singh
B. S. Thippeswami
Ashok D. Taranalli
Anandrao R. KulkarniEmail author

Research Article

First Online:: 27 July 2011

Received:: 21 May 2011
Accepted:: 26 June 2011

DOI: 10.1007/s13596-011-0023-5

Cite this article as:: Ganguly, K., Jagadeesh, N.V., Singh, R. et al. Orient Pharm Exp Med (2011) 11: 177. doi:10.1007/s13596-011-0023-5

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Abstract

This study was performed to determine whether Boswellic acid suppresses genotoxicity induced by cyclophosphamide in mice. Genotoxicity was induced by the administration of cyclophosphamide (50 mg/kg) in swiss albino mice and was measured by counting the number of micronuclei and observing chromosomal aberration. In test group Boswellic acid was given in the dose of 200 mg/kg and various chromosomal aberration and formation of micronuclei were observed. Boswellic acid in dose of 200 mg/kg significantly (P < 0.001) inhibited the cyclophosphamide induced micronucleus formation and also significantly (P < 0.001) inhibited chromosomal aberrations in mice. Whereas Boswellic acid alone did not either induce formation of micronuclei or chromosomal aberration in mice. Boswellic acid itself doesn’t have any genotoxic effect and suppresses genotoxicity induced by cyclophosphamide.

Keywords

Boswellic acid Genotoxicity Micronucleus Chromosomal aberration

References

Adler ID (1984) Cytogenetic tests in mammals. In: Venitt S, Parry JM (eds) Mutagenicity testing: A practical approach. IRL, Oxford, pp 275–306Google Scholar
Andrade N, Perazzo F, Maistro E (2008) Lack of clastogenic/genotoxic effects of Baccharis dracunculifolia extract on Swiss mouse peripheral blood cells. Genet Mol Res 7:1414–1421PubMedCrossRefGoogle Scholar
Andraws R, Chawla P, Brown DL (2005) Cardiovascular effects of ephedra alkaloids: a comprehensive review. Prog Cardiovasc Dis 47:217–225PubMedCrossRefGoogle Scholar
Bishnoi M, Patil CS, Kumar A, Kulkarni SK (2007) Co administration of acetyl-11-keto-beta-boswellic acid, a specific 5-lipooxygenase inhibitor, potentiates protective effect of COX-2 inhibitors in kainic acid induced neurotoxicity in mice. Pharmacol 79:34–41CrossRefGoogle Scholar
Brien JO, Renwick A (2006) Approaches to the risk assessment of genotoxic carcinogens in food: A Critical Appraisal. Food and Chem Toxicol 44:1613–1615CrossRefGoogle Scholar
Burcham PC (1999) Internal hazards Baseline DNA damage by endogeneous products of normal metabolism. Mutat Res 443:11–36PubMedGoogle Scholar
Cuaz PC, Billiet L, Bauge E, Copin C, Scott AD, Genze F, Buchele B, Syrovets T, Simmet T, Rouis M (2008) Anti inflammatory and anti atherogenic effects of NF-kappa B inhibitor acetyl-11- keto-beta-boswellic acid in LPS-challenged ApoE−/−mice. Arterioscler Thromb Vasc Biol 28:272–277CrossRefGoogle Scholar
Ernst E (2002) The risk-benefit profile of commonly used herbal therapies: Ginkgo, St. John’s Wort, Ginseng, Echinacea, Saw Palmetto, and Kava. Ann Intern Med 136:42–53PubMedGoogle Scholar
Gonçalves da Silva RM, Coelho de Sousa N, Graf U, Spano MA (2008) Antigenotoxic effects of Mandevilla velutina (Gentianales, Apocynaceae) crude extract on cyclophosphamide-induced micronuclei in Swiss mice and urethane-induced somatic mutation and recombination in Drosophila melanogaster. Genet Molec Biol 31:751–758CrossRefGoogle Scholar
Hales B (1982) Comparison of the mutagenicity and teratogenicity of Cyclophosphamide and its active metabolites, 4-hydroxycyclophosphamide, phosphoramide mustard and acrolein. Can Res 42:3016–3021Google Scholar
Hayashi M, Tice RR, MacGregor JT, Anderson D, Blakey DH, Kirsh-Volders M, Oleson FB Jr, Pacchierotti F, Romagna F, Shimada H et al (1994) In vivo rodent erythrocyte micronucleus assay. Mutat Res 312:293–304PubMedGoogle Scholar
Hewitt JB (1997) Health effects of occupational exposure to antineoplastic drugs: An integrative research review, Prepared for the occupational disease panel, Ministry of labour, Canada. 1
Huang MT, Badmaev V, Ding Y, Liu Y, Xie JG, Ho CT (2000) Anti-tumor and anti-carcinogenic activities of triterpenoid, β-boswellic acid. BioFactors 13:225–230PubMedCrossRefGoogle Scholar
Krishna G, Hyashi M (2000) In vivo rodent micronucleus assay: protocol, conduct and data interpretation. Mutat Res 455:155–166PubMedCrossRefGoogle Scholar
Krishna G, Petrere J, Anderson J, Theiss J (1995) Use of cyclophosphamide as a positive control in dominant lethal and micronucleus assay. Mutat Res 335:331–337PubMedGoogle Scholar
Lewith GT (2000) Complementary and alternative medicine: an educational, attitudinal and research challenge. Med J Aust 172:102–103PubMedGoogle Scholar
Moore FR, Urda GA, Krishna G, Theiss JC (1995) An in vivo/in vitro method for assessing micronucleus and chromosome aberration induction in rat bone marrow and spleen. 1. Studies with cyclophosphamide. Mutat Res 335:191–199PubMedGoogle Scholar
Prasad S, Naik P, Vijayalaxmi KK (2002) Efficiency of coleus aromaticus extract in modifying cyclophosphamide and mitomycin-c induced clastogenicity in mouse bone marrow cells. Indian J Expt Bio 40:1020–1025Google Scholar
Safayhi H, Mack T, Ammon HP (1999) Protection by Boswellic acids against galactosamine/endotoxin induced hepatitis in mice. Biochem Pharmacol 41:1536–1537CrossRefGoogle Scholar
Sánchez-Lamar A, Fuentes JL, Fonseca G, Capiro N, Ferrer M, Alonzo A, Baluja L, Cozzi R, De Salvia R, Fiore M, Llagostera M (2002) Assessment of the potential genotoxic risk of Phyllantus orbicularis HBK aqueous extract using in vitro and in vivo assays. Toxicol Lett 136:87–96PubMedCrossRefGoogle Scholar
Savage JR (1975) Classification and relationships of induced chromosomal structural changes. J Med Genet 12:103–122Google Scholar
Seyed JH, Mohmmad K (2005) Citrus extract modulates genotoxicity induced by cyclophosphamide in mice bone marrow cells. J Pharm Pharmacol 57:505–509Google Scholar
Sharma ML, Bani S, Singh GB (1989) Antiarthritic activity of Boswellic acids in bovine serum albumin induced arthritis. Int J Immunopharmacol 11:647–652PubMedCrossRefGoogle Scholar
Singh S, Khajuria A, Taneja SC, Khajuria RK, Singh J, Johri RK, Qazi GN (2008) Gastric ulcer protective effect of boswellic acid, leukotriene inhibitor from boswellia serrata in rat. Phytomedicine 15:408–415PubMedCrossRefGoogle Scholar
Swierenga SH, Heddle JA, Sigal EA, Gilman JP, Brillinger RL, Douglas GR, Nestmann ER (1991) Recommended protocol based on a survey of current practice in genotoxicity testing laboratories. IV. Chromosome aberrations and sister chromatid exchange in chinese hamster ovary V79 chinese hamster lung and human lymphocytes cultures. Mutat Res 246:301–322PubMedCrossRefGoogle Scholar

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Authors and Affiliations

Kuntal Ganguly
- 1
N. V. Jagadeesh
- 1
Rakesh Singh
- 1
B. S. Thippeswami
- 1
Ashok D. Taranalli
- 2
Anandrao R. Kulkarni
- 1
Email author

1.Department of PharmacologySET’s College of PharmacyDharwadIndia
2.KLE University’s College of PharmacyBelgaumIndia

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Boswellic acid suppresses cyclophosphamide induced chromosomal damage in mice

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