Stereologic Analysis of Tissue Compartments of Gunshot-injured and Blunt-injured Spleen Novica M. Milićević Jasna B. Trbojević-Stanković Cinthia B. Drachenberg Živana Milićević Email author Article First Online: 09 August 2009 Received: 20 April 2009 Accepted: 09 July 2009 DOI :
10.1007/s12253-009-9189-2
Cite this article as: Milićević, N.M., Trbojević-Stanković, J.B., Drachenberg, C.B. et al. Pathol. Oncol. Res. (2010) 16: 69. doi:10.1007/s12253-009-9189-2 Abstract The spleen is composed of several tissue compartments and the respective histoquantitative data are essential for complete understanding of immune or pathological processes in this organ. The aim of our study was to determine and compare the stereologic parameters of all tissue compartments of the gunshot-injured and blunt-injured human spleen. The model-based stereology with point-counting method was utilized to study the volume densities of red pulp, perifollicular zone, marginal zone, white pulp (follicles and periarteriolar lymphoid sheath), and connective tissue. The areal numerical density (the number of follicles per mm2 of tissue section), the numerical density (the number of follicles per mm3 of tissue) of lymphoid follicles and the mean follicle diameter were also determined. Our study provides stereological parameters for all tissue compartments of the human spleen. No morphometric differences were registered between tissue compartments of the blunt-injured and gunshot-injured spleen. As the gunshot-injured spleen was taken as presumably unstimulated in immunological regard, our results suggest that both gunshot-injured and blunt-injured organs may be used as models of the normal human spleen.
Keywords Blunt-injured spleen Gunshot-injured spleen Human Morphometry Stereology Splenic tissue compartments Abbreviations PALS periarteriolar lymphoid sheath
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Authors and Affiliations Novica M. Milićević Jasna B. Trbojević-Stanković Cinthia B. Drachenberg Živana Milićević Email author 1. Institute of Histology and Embryology Faculty of Medicine Belgrade Serbia 2. Department of Hemodialysis Clinical Centre “Dr Dragiša Mišović” Belgrade Serbia 3. Department of Pathology University of Maryland School of Medicine Baltimore USA