An Immunohistochemical Study of Colon Adenomas and Carcinomas: E-cadherin, Syndecan-1, Ets-1

Zsuzsanna Pap; Zoltán Pávai; Lóránd Dénes; Ilona Kovalszky; János Jung

doi:10.1007/s12253-009-9157-x

Pathology & Oncology Research

December 2009, 15:579

An Immunohistochemical Study of Colon Adenomas and Carcinomas: E-cadherin, Syndecan-1, Ets-1

Authors
Authors and affiliations

Zsuzsanna PapEmail author
Zoltán Pávai
Lóránd Dénes
Ilona Kovalszky
János Jung

Original Paper

First Online:: 01 March 2009

Received:: 02 December 2008
Accepted:: 17 February 2009

DOI: 10.1007/s12253-009-9157-x

Cite this article as:: Pap, Z., Pávai, Z., Dénes, L. et al. Pathol. Oncol. Res. (2009) 15: 579. doi:10.1007/s12253-009-9157-x

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Abstract

It is thought that dysregulation of E-cadherin, syndecan-1 (CD138) and Ets-1 is involved in carcinoma development. E-cadherin is an important epithelial cell adhesion molecule; syndecan-1 (CD138) is a regulatory proteoglycan in both cell-cell and cell-matrix adhesion and Ets-1 is a proto-oncogene and transcription factor, which takes part in extracellular matrix remodeling. Our goal was to study the changes in the expression of these molecules during colon carcinoma development and progression. We tested 117 colon adenomas and 149 de novo and ex adenoma carcinomas of the colon, using the Ultravision Polymer system. The positive reaction rate was 100% for E-cadherin, 98.3% for syndecan-1 and 22.4% for Ets-1 in adenomas, while in carcinomas it was 88.5%, 62.4% and 56.3% respectively. We found decreasing expression of E-cadherin and syndecan-1 throughout colon carcinoma progression and an opposite regulation for the Ets-1 protein. Decrease in expression of syndecan-1 is more pronounced in carcinomas compared to E-cadherin. De novo carcinomas have lower E-cadherin and syndecan-1 expression, and higher Ets-1 expression compared to ex adenoma carcinomas. These findings support the hypothesis that there are differences in the carcinogenesis of these tumors.

Keywords

Colon adenoma Colon carcinoma E-cadherin Ets-1 Immunohistochemistry Syndecan-1

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Authors and Affiliations

Zsuzsanna Pap
- 1
Email author
Zoltán Pávai
- 1
Lóránd Dénes
- 1
Ilona Kovalszky
- 2
János Jung
- 3

1.Department of Anatomy and EmbryologyUniversity of Medicine and Pharmacy Târgu-MureşTârgu-MureşRomania
2.1st Institute of Pathology and Experimental Cancer ResearchSemmelweis University of MedicineBudapestHungary
3.Department of PathologyUniversity of Medicine and Pharmacy Târgu-MureşTârgu-MureşRomania

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An Immunohistochemical Study of Colon Adenomas and Carcinomas: E-cadherin, Syndecan-1, Ets-1

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