Microvessel count, proliferating cell nuclear antigen and Ki-67 indices in gastric adenocarcinoma Gülsüm Özlem Elpek Email author Tekinalp Gelen Nazif Hikmet Aksoy Tuncer Karpuzoğlu Nuran Keles Article Received: 30 November 1999 Accepted: 20 January 2000 DOI :
10.1007/BF03032660
Cite this article as: Elpek, G.Ö., Gelen, T., Aksoy, N.H. et al. Pathol. Oncol. Res. (2000) 6: 59. doi:10.1007/BF03032660 Abstract The aim of the present study was to immunohistochemically investigate the prognostic value of neovascularization (expressed as microvessel count-MVC) and tumor cell proliferation (expressed as PCNA labeling index - PLI and Ki-67 labeling index- KLI) in gastric adenocarcinoma. Correlations with clinicopathologic features were also evaluated. Tumor specimens from 74 patients diagnosed as gastric adenocarcinoma were included in this study. For-malin fixed, paraffin embedded tissue sections stained immunohistochemically with F-VIII, PC10 and MIB-1 monoclonal antibodies. By ocular grid subdivided into 100 areas, number of microvessels and PC10, MIB-1 positive and negative cells were counted at x400 magnification. Chi-square test, Kaplan-Meier method and cox regression analysis were used for statistical analysis. The results showed that, MVC and PLI had a significant correlation with invasion and lymph node metastasis. The prognosis was significantly worse in patients with high MVC (>14) and with high PLI (>49%). However any relationship was not observed between KLI (38%) and clinicopathologic parameters, so KLI failed to predict the prognosis. Cox model showed that, MVC and PLI were independent prognostic variables. Ki-67 labeling index in gastric carcinomas has no prognostic relevance. However, the evaluation of microvessel count and proliferating cell nuclear antigen index in gastric carcinomas could be reliable indicators of prognosis.
Keywords microvessel count angiogenesis proliferation gastric carcinoma Abbrevations MVC Microvessel count
PLI Proliferating cell nuclear antigen labeling index
KLI Ki-67 labeling index
This work was presented at the 17th European Congress of Pathology, Barcelona, Spain, September 18–23, 1999.
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© Arányi Lajos Foundation 2000
Authors and Affiliations Gülsüm Özlem Elpek Email author Tekinalp Gelen Nazif Hikmet Aksoy Tuncer Karpuzoğlu Nuran Keles 1. Department of Pathology Akdeniz University, Medical School Antalya Turkey 2. Department of General Surgery Akdeniz University, Medical School Antalya Turkey 3. Týp Fakültesi Patoloji ABD Akdeniz Üniversitesi Antalya Turkey