A new method to localize acid phosphatase using the confocal laser-scanning microscope

Zsolt Szegedi; Farhana Amin; Béla Szende; Ivor Delme Bowen

doi:10.1007/BF02905252

Pathology & Oncology Research

September 1998, Volume 4, Issue 3, pp 217–223

A new method to localize acid phosphatase using the confocal laser-scanning microscope

Authors
Authors and affiliations

Zsolt SzegediEmail author
Farhana Amin
Béla Szende
Ivor Delme Bowen

Methods

Received:: 25 July 1998
Accepted:: 02 September 1998

DOI: 10.1007/BF02905252

Cite this article as:: Szegedi, Z., Amin, F., Szende, B. et al. Pathol. Oncol. Res. (1998) 4: 217. doi:10.1007/BF02905252

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Abstract

The aim of the study was to develop a technique for the detection of acid phosphatase enzyme activity by confocal laser-scanning microscope using the histochemical acid phosphatase detection method (after Barka and Anderson 1962, modified by Bowen and Lewis 1985) routinely used for light microscopy. The density and the distribution of enzyme reaction product is dependent on the incubation time, as shown by different confocal images or ELISA reader. The inhibition of the enzyme activity with metal ions shows the same profile known from the literature. This staining method seems to be useful to demonstrate subcellular distribution of the enzyme in the lysosomes and in the Golgi apparatus.

Key words

acid phosphatase activity cytometry cell image analysis confocal laser-scanning microscope

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Authors and Affiliations

Zsolt Szegedi
- 1
- 3
Email author
Farhana Amin
- 2
Béla Szende
- 1
Ivor Delme Bowen
- 2

1.1st Institute of Pathology and Experimental Cancer Research, Department of Molecular PathologyJoint Research Organization of the Hungarian Academy of Sciences and Semmelweis University of MedicineBudapestHungary
2.Department of Pure and Applied BiologyUniversity of CardiffCardiffUK
3.BudapestHungary

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A new method to localize acid phosphatase using the confocal laser-scanning microscope

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