항산화 활성과 타이로시나제 억제 활성을 갖는 카페익산-우유단백질 유래 펩타이드에 관한 연구

항산화제;타이로시나제;카페익산;우유 단백질;합성;항산화 활성;미백 활성;660

공과대학 화학생물공학부 ;

University:서울대학교 대학원

关键词: 항산화제;    타이로시나제;    카페익산;    우유 단백질;    합성;    항산화 활성;    미백 활성;    660;   

Others  :  http://s-space.snu.ac.kr/bitstream/10371/129396/1/000000131908.pdf

美国|英语

来源: Seoul National University Open Repository

【 摘 要 】

Anti-aging and depigmentation has been the subject of much attention in pharmaceutical and cosmetic industries. Antioxidants distributed in various natural plants such as fruits, tea and coffee have been widely studied as an agent against reactive oxygen species (ROS)-related diseases as well as cosmetic ingredients. Especially, phenolic antioxidant family, such as caffeic acid (CA), shows strong antioxidant activity via stabilization of phenoxy radical from its fully conjugated structure after scavenging of ROS. Skin-lightening agents have been also found in various natural source including milk-derived protein, which suppress melanin production by means of tyrosinase inhibition activity. We prepared dual effector molecules consisting of CA and MHIRL derived peptides (MDPs: MHIR, HIRL, HIR), which shows both activities of antioxidant and tyrosinase inhibition. CA-MHIRL derived peptides (CA-MDPs) were synthesized by conventional solid-phase peptide synthesis (SPPS), and characterized by RP-HPLC and ESI-MS. Synthetic efficiency was improved through the protection of reactive hydroxyl groups of CA and the optimization of cleavage condition for successful separation of the product from solid support without any side reaction. As-synthesized CA-MDPs, especially CA-MHIR, showed good antioxidant activity and highly enhanced tyrosinase inhibitory activity in various assay systems. In mushroom tyrosinase inhibition test, CA-MHIR exhibited about 4 times lower IC₅₀ values (48 μM) than kojic acid, one of the most popular tyrosinase inhibitor (201 μM). Kinetic study of the inhibition by CA-MHIR revealed that CA-MHIR is regarded as a non-competitive inhibitor of mushroom tyrosinase. In addition, CA-MDPs exhibited no-cytotoxicity in melanoma cell, B16-F1. We considered that CA-MHIR dual effector could be applied in the fields of cosmetic industry.