In Vitro Selection of mRNA-Display Libraries Containing Unnatural Amino Acids

chemical derivatization;mRNA-display libraries;non-sense suppression;unnatural amino acids

Li, Shuwei ; Roberts, Richard W.

University:California Institute of Technology

Department:Chemistry and Chemical Engineering

关键词: chemical derivatization;    mRNA-display libraries;    non-sense suppression;    unnatural amino acids;   

Others  :  https://thesis.library.caltech.edu/1853/1/Acknowledgements.pdf

美国|英语

来源: Caltech THESIS

【 摘 要 】

The mRNA display library provides a strategy to make up to 10[superscript 13] unique peptides that fuse with their own genes via a covalent link.In this thesis, several strategies to prepare mRNA display libraries containing unnatural side chains are described.Chapter 1 is a short introduction and application of mRNA-display technology.Chapter 2 describes a chemical derivatization strategy to append an unnatural side chain to libraries.Chapter 2 introduces the incorporation of unnatural amino acids via non-sense codon suppression.Chapter 4 focuses on the catalytic selection with mRNA display libraries.These libraries should facilitate the discovery of novel ligands with functionalities beyond those provided by the 20 naturally occurring amino acids.