Cartilage tissue provides compressive resistance in diarthrodial joints, and hasbeen shown to be regulated by mechanical signals, in particular with regard to productionof extracellular matrix proteins. However, less is understood about how chondrocytes inregions not solely purposed to provide compressive resistance may also be affected bymechanical forces. The growth plate is a small layer of cartilage that functions tofacilitate longitudinal growth of the long bones from in utero through post-adolescentdevelopment. The growth plate maintains distinct regions of chondrocytes at carefullyregulated stages of endochondral ossification that are in part characterized by theirmorphology and differential responsiveness to vitamin D metabolites. Understanding ifmechanical cues could be harnessed to accelerate or delay the process of endochondralossification might be beneficial for optimizing tissue engineering of cartilage orosteochondral interfaces. This study focused on three aims to provide a basis for futurework in this area: 1) Develop a cell line culture model useful for studying growth platechondrocytes, 2) Determine the response of primary growth plate chondrocytes and thecell line model to fluid shear stress, and 3) determine if expression of integrin beta 1 isimportant for the observed responses to shear stress. The findings of this study suggestthat inorganic phosphate can promote differentiation in coordination with the24,25(OH)2D3 metabolite of vitamin D, and that fluid shear stress generally inhibitsdifferentiation and proliferation of growth plate chondrocytes in part through an integrinbeta 1 mediated pathway.
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The effect of fluid shear stress on growth platechondrocytes
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