Ultra-high resolution DNA crystallography

Crystallography;Intercalators;Cations;DNA

Maehigashi, Tatsuya ; Chemistry and Biochemistry

University:Georgia Institute of Technology

Department:Chemistry and Biochemistry

关键词: Crystallography;    Intercalators;    Cations;    DNA;   

Others  :  https://smartech.gatech.edu/bitstream/1853/29684/1/maehigashi_tatsuya_200908_phd.pdf

美国|英语

来源: SMARTech Repository

【 摘 要 】

This thesis represents results of experiments involved in ultra-high resolution x-ray crystal structures of DNA. Detailed analysis of DNA coordinates of increased accuracy, refinement of individual anisotropic B-factors, visualization of disorder, counterions and hydration are presumably facilitated by the increased information of ultra-high resolution structures. However, recent survey of available DNA crystal structures with the data that extends beyond 1Ã... has brought a few areas of considerations; local conformational flexibility of DNA and counterions and how they have been interpreted in previous studies. While the results given here demonstrate both structural and surrounding details of DNA that may be undetected at lower resolution, some of the technical aspects of interpreting ultra-high resolution data is also addressed.