Efficacy of Immersion and Intraperitoneal Vaccination Against Yersinia Ruckeri and the Effects on Immune Response in Rainbow Trout, Oncorhynchus Mykiss

rainbow trout;vaccination;yersinia ruckeri

Jenkins, Thomas Burton II ; Dr. David A. Dickey, Committee Member,Dr. Michael G. Levy, Committee Member,Dr. Jeffrey M. Hinshaw, Committee Chair,Jenkins, Thomas Burton II ; Dr. David A. Dickey ; Committee Member ; Dr. Michael G. Levy ; Committee Member ; Dr. Jeffrey M. Hinshaw ; Committee Chair

University:North Carolina State University

关键词: rainbow trout;    vaccination;    yersinia ruckeri;   

Others  :  https://repository.lib.ncsu.edu/bitstream/handle/1840.16/861/etd.pdf?sequence=1&isAllowed=y

美国|英语

来源: null

【 摘 要 】

In this study, the effectiveness of vaccination against Yersinia ruckeri, using an autogenous bacterin, in rainbow trout (Oncorhynchus mykiss) was assessed.In addition, the immune response detected by enzyme-linked immunosorbent assay (ELISA) was evaluated.Rainbow trout were vaccinated at 25 grams mean body weight by standard bath immersion and intraperitoneal (i.p.) injection (saline-based injection or oil-based adjuvant injection). Bath-challenges occurred on days 42, 101, 181, 286, and 381 post-vaccination.Plasma samples were collected pre-challenge and fourteen days post-challenge for antibody titer analysis.Antibody kinetics were followed prior to first challenge.Relative percent survival (RPS) ranged from 98.96% to 96.85% and did not statistically differ among vaccinated groups.Increased antibody titer was identified in the oil-based adjuvant injection vaccinated group however, statistical significance among vaccinated groups was not determined.