Efficacy of Immersion and Intraperitoneal Vaccination Against Yersinia Ruckeri and the Effects on Immune Response in Rainbow Trout, Oncorhynchus Mykiss
rainbow trout;vaccination;yersinia ruckeri
Jenkins, Thomas Burton II ; Dr. David A. Dickey, Committee Member,Dr. Michael G. Levy, Committee Member,Dr. Jeffrey M. Hinshaw, Committee Chair,Jenkins, Thomas Burton II ; Dr. David A. Dickey ; Committee Member ; Dr. Michael G. Levy ; Committee Member ; Dr. Jeffrey M. Hinshaw ; Committee Chair
In this study, the effectiveness of vaccination against Yersinia ruckeri, using an autogenous bacterin, in rainbow trout (Oncorhynchus mykiss) was assessed.In addition, the immune response detected by enzyme-linked immunosorbent assay (ELISA) was evaluated.Rainbow trout were vaccinated at 25 grams mean body weight by standard bath immersion and intraperitoneal (i.p.) injection (saline-based injection or oil-based adjuvant injection). Bath-challenges occurred on days 42, 101, 181, 286, and 381 post-vaccination.Plasma samples were collected pre-challenge and fourteen days post-challenge for antibody titer analysis.Antibody kinetics were followed prior to first challenge.Relative percent survival (RPS) ranged from 98.96% to 96.85% and did not statistically differ among vaccinated groups.Increased antibody titer was identified in the oil-based adjuvant injection vaccinated group however, statistical significance among vaccinated groups was not determined.
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Efficacy of Immersion and Intraperitoneal Vaccination Against Yersinia Ruckeri and the Effects on Immune Response in Rainbow Trout, Oncorhynchus Mykiss