Binding and Local Dynamics of Iso-1-cytochrome c to Substrate-Supported Lipid Nanotube Arrays

spin-labeling EPR

Smith, Shani Jomella ; Edmond Bowden, Committee Member,Alex Smirnov, Committee Chair,Tatyana Smirnova, Committee Member,Smith, Shani Jomella ; Edmond Bowden ; Committee Member ; Alex Smirnov ; Committee Chair ; Tatyana Smirnova ; Committee Member

University:North Carolina State University

关键词: spin-labeling EPR;   

Others  :  https://repository.lib.ncsu.edu/bitstream/handle/1840.16/108/etd.pdf?sequence=1&isAllowed=y

美国|英语

来源: null

【 摘 要 】

A study of the binding and local dynamics of iso-1-cytochrome c to lipid nanotube arrays composed of 1,2-Diheptadecanoyl-sn-Glycero-3-[Phospho-rac-(1-glycerol)] (DOPG), an anionic phospholipid on an nanoporous anodic aluminum oxide (AAO) substrate was undertaken to gain an understanding of the effects of nanoscale confinement on protein binding.Iso-1-cytochrome c was spin-labeled with 1-oxyl-2,2,3,3-tetramethylpyrolline-3-methylmethanesulfonate (MTSL) at cysteine 102. The binding and local dynamics of the spin-labeled iso-1-cytochrome c bound to multilamellar liposomes was compared with the binding to lipid nanotube arrays.Location of spin-labeled cysteine 102 of iso-1-cytochrome c with respect to the bilayer was probed using an EPR oxygen accessibility experiment.CD monitored changes in the heme coordination in iso-1-cytochrome c upon binding to DOPG.