Analysis of microRNA microarray (MM chip) data for aging mice models.

MicroRNA microarray;Normalization techniques;Repeated measures;Aging mice models

Vikranth Shetty

University:University of Louisville

Department:Bioinformatics and Biostatistics

关键词: MicroRNA microarray;    Normalization techniques;    Repeated measures;    Aging mice models;   

Others  :  https://ir.library.louisville.edu/cgi/viewcontent.cgi?article=2317&context=etd

美国|英语

来源: The Universite of Louisville's Institutional Repository

【 摘 要 】

The present experimental designs have unique challenges in the analysis of microRNAexpression intensity levels. In this project, various Normalization techniques werediscussed with different statistical techniques and their application in an analysisperformed at the Genes Centre of aging on mice models. The project involved repeatedmeasures over time, and the intra-subject correlation caused by the repeatedmeasurements should be incorporated into the analysis, resulting in additional significantmiRNAs. Collection of multiple samples from the same individuals over time from anexperiment may have issues with the commonly used methodologies-these issues arediscussed here. Mixed model approach is used here to see the difference betweendifferent normalized data with the raw data with repeated measurements.