Using photonic crystal biosensors for pharmaceutical screening

Label-free;Biosensor

George, Sherine ; Cunningham, Brian T. ; Cunningham ; Brian T.

关键词: Label-free;    Biosensor;   

Others  :  https://www.ideals.illinois.edu/bitstream/handle/2142/14723/George_Sherine.pdf?sequence=2&isAllowed=y

美国|英语

来源: The Illinois Digital Environment for Access to Learning and Scholarship

【 摘 要 】

The application of photonic crystal surfaces for label-free bioimaging and sensing is presented. A label-free biosensor assay is used to monitor the density of cell attachment to thesensor surface, and to quantify changes in attached cell density induced by the introduction ofplant extracts. This image-based biosensor detection approach can effectively identify plantextracts with cytotoxic effects on cancer cells using only 500 cells per test. A library of sixty-oneplant extract from Bangladesh is studied. We have also demonstrated that cell death in extracttreatedcells occurs via apoptosis. Next, we take a closer look at the cytotoxicity of extracts froma single genus – the Amoora genus, on a panel of five different cancer cell lines. The effect of the extraction method used in preparing the extract sample on cell cytotoxicity is explored. Anongoing project involving the screening of plant protein samples from Pakistan is then presented.In further work, the description of the photonic crystal biosensor used for a protein macromolecule binding assay is presented. A high-throughput screen aimed at identifying small molecule disrupters of protein-DNA binding is designed and executed. Both the cell-based assay and the protein-DNA based assay described here find important applications in the modern drug discovery process.