Hepatic gene networks due to the trans-10, cis-12-conjugated linoleic acid supplementation in growing male mice

Conjugated linoleic acid;hepatic steatosis

Li, Jing ; Loor ; Juan J.

关键词: Conjugated linoleic acid;    hepatic steatosis;   

Others  :  https://www.ideals.illinois.edu/bitstream/handle/2142/24310/Li_Jing.pdf?sequence=1&isAllowed=y

美国|英语

来源: The Illinois Digital Environment for Access to Learning and Scholarship

【 摘 要 】

Trans-10, cis-12-conjugated linoleic acid (trans-10, cis-12-CLA) has been reported to reduce body fat mass but accompanied by increased liver mass. However, the cellular and molecular mechanisms have been unknown. Our study investigated the effects of trans-10, cis-12-CLA supplementation on hepatic gene expression in growing male mice by measuring mRNA abundance of thirty-eight genes encoding important enzymes, ligand-dependent nuclear receptors, transcription regulators and transporters in lipid metabolism. Feeding growing male mice diets supplemented with 0.3% trans-10, cis-12-CLA for 6 weeks significantly increased the expression of hepatic genes involved in fatty acid uptake (Cd36), TG synthesis (Acaca, Gpam and Scd), lipid droplet formation (Plin2), VLDL assembling/secretion (Mttp and Cideb), ketogenesis and utilization (Hmgcs2 and Bdh1), fatty acid oxidation (Acox1), glyceroneogenesis (Pck1 and Pdk4). The results suggest that the increased liver weight in response to trans-10, cis-12-CLA may be due to increased fatty acid uptake, stimulated TG synthesis, enhanced formation of lipid droplets as well as insufficiently induced VLDL assembly/secretion mechanism in liver. We provided new evidences to advance in the knowledge of the effect of this supplement in a relatively low amount on hepatic gene expression in growing male mice.