Mid infrared spectroscopic imaging and tomography

Fourier Transform Infrared (FT-IR) spectroscopic Imaging;Mid-Infrared Spectroscopic Tomography;Spectroscopic Imaging;Spectroscopic Tomography;mid-Infrared (Mid-IR);Spectroscopy;Imaging

Reddy, Rohith

关键词: Fourier Transform Infrared (FT-IR) spectroscopic Imaging;    Mid-Infrared Spectroscopic Tomography;    Spectroscopic Imaging;    Spectroscopic Tomography;    mid-Infrared (Mid-IR);    Spectroscopy;    Imaging;   

Others  :  https://www.ideals.illinois.edu/bitstream/handle/2142/44759/Rohith_Reddy.pdf?sequence=1&isAllowed=y

美国|英语

来源: The Illinois Digital Environment for Access to Learning and Scholarship

【 摘 要 】

Fourier Transform Infrared (FT-IR) Spectroscopic Imaging is an optical technique that simultaneously provides morphological (two dimensional) and mid-infrared spectral (one dimensional) information for a specimen under observation. In this dissertation, we develop a new instrument that extends the capabilities of FT-IR imaging to provide three dimensional morphological information along with mid-infrared spectral information on the microscopic length scale. We first demonstrate that spectroscopic information from the mid-infrared can be used to perform histologic recognition and cancer detection in prostate, breast and skin cancer tissues. These studies utilize FT-IR imaging data of thin tissue slices from several hundred patients. The segmentation algorithms and instrumentation used are described in detail. We perform a rigorous analysis of instrumentation and data so as to understand the limits and limitations of current state-of-the-art instrumentation. We then propose a new instrument that adds a new dimension of information to current instruments and establish the new state-of-the-art in mid-infrared imaging. The theoretical basis of such instrumentation is presented in detail and techniques for visualization of four dimensional data are outlined.