A spectroscopic smartphone biosensor for use in point-of-care diagnostic applications

Smartphone Biosensing;Photonic Crystal;Enzyme-Linked Immunosorbent Assay;Mobile Biosensing;Point-of-Care Diagnostics

Long, Kenneth ; Cunningham ; Brian T.

关键词: Smartphone Biosensing;    Photonic Crystal;    Enzyme-Linked Immunosorbent Assay;    Mobile Biosensing;    Point-of-Care Diagnostics;   

Others  :  https://www.ideals.illinois.edu/bitstream/handle/2142/49844/Kenneth_Long.pdf?sequence=1&isAllowed=y

美国|英语

来源: The Illinois Digital Environment for Access to Learning and Scholarship

【 摘 要 】

This thesis serves to introduce a new point-of-care testing device capable of providing spectrometric data for solid and liquid phase samples.A custom cradle was developed to maintain optical alignment allowing for the diffraction, and therefore spectrometric analysis, of light transmitted either directly through a sample or via an intermediary optical biosensor.Demonstration of the instrument is provided with both an enzyme-linked immunosorbent assay (ELISA) and a photonic-crystal (PC)-based investigation of biomolecular adsorption.ELISA analysis is completed on both human interleukin-6, an important diagnostic cancer biomarker, and Ara h 1, one of the principle peanut proteins responsible for allergic reactions.PC-based sensing was completed with a Protein A-immunoglobulin G interaction with a .009 nm accuracy.All of these demonstrations were completed at physiologically-relevant concentrations, and as such, serve as proof-of-concept for two separate modalities of a smartphone-based spectrometric optical biosensor.In the future, such a sensor has the potential to provide a low-cost, point-of-care diagnostic device that is inherently robust, user-friendly, cloud-connected, and suited for use in remote sensing applications.