Mueller-matrix-based analysis for second-harmonic generation imaging

Second-harmonic generation;Mueller matrix

Okoro, Chukwuemeka Onyekere

关键词: Second-harmonic generation;    Mueller matrix;   

Others  :  https://www.ideals.illinois.edu/bitstream/handle/2142/78681/OKORO-THESIS-2015.pdf?sequence=1&isAllowed=y

美国|英语

来源: The Illinois Digital Environment for Access to Learning and Scholarship

【 摘 要 】

Second-harmonic generation microscopy takes advantage of second-order nonlinear property for imaging of biological structures. Invoking quantitative image analysis techniques facilitates the assessment of tissues in varying states.This thesis presents a quantitative description of the relative structural difference in collagen-based tissues by second harmonic generation (SHG) imaging techniques involving polarization investigation. The Mueller matrix formalism demonstrates a polarization-based analysis approach for assessment of varying samples.The associated second-order Mueller matrices are generated by applyingimage processing algorithms to sub-grids of image sets obtained from a forward imaging microscope. By defining scalar metrics such as the degree of polarization and the depolarization, a quantitative evaluation of the samples under investigation is provided. A discussion of the capabilities for tissue assessment and techniques for improvements in the data acquisition speed and analysis will also be proffered