Engineering and optimizing physiological cell isolation via the secondary anchor targeted cell release system

Surface Functionalization, Microfluidics, Cell Isolation, Streptavidin, Biotin, Cell Patterning

Ansari, Ali

关键词: Surface Functionalization, Microfluidics, Cell Isolation, Streptavidin, Biotin, Cell Patterning;   

Others  :  https://www.ideals.illinois.edu/bitstream/handle/2142/100979/ANSARI-DISSERTATION-2018.pdf?sequence=1&isAllowed=y

美国|英语

来源: The Illinois Digital Environment for Access to Learning and Scholarship

【 摘 要 】

Cancer treatment regimens, such as chemotherapies are fundamentally limited through patient drug resistance, as patients respond differentially due to these individualized resistances and differences in biomarker expression on cells. Quantification of these biomarkers, then, would allow a methodology for designing personalized treatments and regimens that the patients would no longer be resistant to. However, techniques designed to purify or isolate cells to quantify these biomarkers are not designed to maintain physiological cell expression. In order to develop an isolation modality to preserve receptor numbers, I have developed and optimized the Secondary Anchor Targeted Cell Release (SATCR) system to separate out cells of interest for downstream analysis. The SATCR enables both capture and release of cells through the targeting of the secondary anchor- streptavidin- through the introduction of 4mM biotin into the system. The system has been optimized to preserve physiological wall shear stress, receptor quantity and cell diameter of cells isolated through the system. This allows for our system to create a more physiologically faithful modality for downstream analysis- potentially opening the door to more physiological analyses of purified cell samples for personalized medicine.Surface functionalization allows for the customization and adaption of surfaces for a variety of needs and applications. We have used surface functionalization to adapt glass and PDMS surfaces with the SATCR surface, but there exists a great deal of mineable space for surface functionalization and its adoption in existing modalities. This space includes moving the SATCR surface from static glass based systems into dynamic microfluidic glass and PDMS systems, and possibly even further to non-standard functionalized materials such as polyvinyl chloride. The functionalization of alternate materials would allow further customization and easier adoption of the capture surface into other substrates, further increasing the utility and degrees of freedom for the SATCR capture surface. In addition to substrate alteration, further adaptions and modifications can advance and optimize the SATCR technology to enable more effective and selective isolation of cells through SATCR integration.