The Effect of Different TALP-Components on the Progressive Motility of Bovine Spermatozoa During the Centrifugation and Incubation Stages as a Prerequisite for In Vitro Fertilisation

Veterinary science

Kinis, Athanasios K

University:University of Glasgow

关键词: Veterinary science;   

Others  :  http://theses.gla.ac.uk/77687/1/10997959.pdf

来源: University of Glasgow

【 摘 要 】

In the present study the effect of different Modified Tyrode's media-TALP and different sperm concentrations on the progressive motility of the spermatozoa was examined. TALP medium containing high levels of lactate, NaHCO3 and Hepes provided the best environment for the spermatozoa during the swim-up first and second centrifugation stages. However the medium, in which lactate was the primary nutritional source, gave the best results during CO2 incubation. Treatments of spermatozoa in an air environment gave much poor results than treatments in a CO2 environment. Sperm motility was optimal when the sperm concentration was around 20x10.