Studies on a Temperature-Sensitive Mutant of Feline Calcivirus

Veterinary science

Al-Fallouji, Hana K. A

University:University of Glasgow

关键词: Veterinary science;   

Others  :  http://theses.gla.ac.uk/77343/1/10991715.pdf

来源: University of Glasgow

【 摘 要 】

A temperature sensitive (ts) mutant of feline calicivirus (FCV) was isolated from a vaccine strain of FCV. Virus-infected feline embryo cells were treated with the mutagen 5-fluorouracil (5-FU). The resulting virus was cloned and 192 clones were tested for growth at 31