Microarray analysis of chromosome 14 congenic strains in the SHRSP

RC Internal medicine;QH426 Genetics

Crawford, Wendy ; McBride, Martin

University:University of Glasgow

Department:Institute of Cardiovascular and Medical Sciences

关键词: Microarray, Congenic, Molecular;   

Others  :  http://theses.gla.ac.uk/2761/1/2010CrawfordwMSc.pdf

来源: University of Glasgow

【 摘 要 】

The stroke-prone spontaneously hypertensive rat (SHRSP) is an excellent inbred model of cardiovascular disease. Previous work in our laboratory utilising chromosome 14 congenic strains confirmed a quantitative trait locus for left ventricular mass index.The aims of this project were to use gene expression profiling in the heart during the development of left ventricular hypertrophy (LVH) to identify positional candidate genes within the congenic interval. Exon analysis identified significant differential expression of Chemokine ligand 13 (Cxcl13) in 5 week old SHRSP, WKY and respective chromosome 14 congenic strains.After validation by qRT-PCR, DNA sequencing and Transfac analysis implicated the loss of Hepatocyte Nuclear Factor 4 in the SHRSP.Illumina analysis identified differential expression of Osteopontin (Spp1) in neonatal hearts of the SHRSP versus WKY and respective chromosome 14 congenic strains as a positional candidate gene.The identification of these positional genes prior to the onset of hypertension may identify novel mechanisms in the development of LVH in the SHRSP.