Novel uses of spatial light modulators in optical tweezers

Q Science (General);QR Microbiology;QC Physics

Preece, Daryl ; Padgett, Miles J.

University:University of Glasgow

Department:School of Physics and Astronomy

关键词: spatial light modulators, SLM, Optical trapping, optical tweezers, OSLM, ESLM, micro-rheology, Tweezers software, particle tracking,rotational micro-rheology, Vaterite, micro-macheines, viscosity measurement.;   

Others  :  http://theses.gla.ac.uk/2619/1/2011preecephd.pdf

来源: University of Glasgow

【 摘 要 】

In recent years spatial light modulators (SLMs) have become an integral part of many optical trapping experiments. Yet their usefulness, which stems from their flexibility, is often under exploited. In this thesis I seek to demonstrate how it is possible to expand the range of optical trapping applications that may benefit from the use of spatial light modulators. From exploring the benefits of increased resolution to demonstrating novel applications like position clamping and polarization control, I show how SLMs are a resource which can benefit optical trapping in new and unconventional ways. The optical properties of liquid crystals have long been known however it is only recently that they have been applied to optical tweezers. The physics and operation of spatial light modulators are discussed in chapter 1, with specific attention paid to those aspects of operation which are of pertinent practical use to optical trapping. In chapter 2 it is shown how phase only modulation can be used to create effective holographic optical tweezers systems which are capable of manipulating micron scale particles and measuring pico-Newton forces. Chapter 3 charts the development and characterization of a 4 Mega-pixel spatial light modulator which was created as an improvement on current SLM technology. The role of SLMs in utilising lights angular momentum as a tool for creating rotational torque is discussed in chapter 4. In chapter 5 describes how SLMs can be used to create torques based the application of spin angular momentum to birefringent particles. We show, in chapter 6 how with suitable software engineering it is possible to both move optical traps and track particles in real time. Since the use of SLMs has been previously been limited by their bandwidth constraints we discuss in chapter 7 the use spatial light modulators in closed loop systems. We finish with a discussion of the use of SLMs in a new technique that may be applied to microrheology.