Investigation of 18F-Fluoro-L-Thymidine to monitor treatment response in murine models of pancreatic cancer: development of tools and validation

QC Physics;RC0254 Neoplasms. Tumors. Oncology (including Cancer)

Pajak, Malgorzata Zofia ; Anderson, Kurt I

University:University of Glasgow

Department:Institute of Cancer Sciences

关键词: PET, CT, Albira, NEMA NU4, pancreatic cancer, PDAC, mouse model, KPC, KC Pten.;   

Others  :  http://theses.gla.ac.uk/8093/1/2016pajakPhD.pdf

来源: University of Glasgow

【 摘 要 】

I characterised performance of the Positron Emission Tomography (PET) and the Computed Tomography (CT) modules of the 2-ring Albira PET/SPECT/CT, a small-animal imaging platform. The evaluation of PET was done in concordance with the National Electrical Manufacturer’s Association (NEMA) NU4-2008 standard. The performance of the Albira CT was assessed using microCT phantom. As a way of verification of the results of the phantom studies, example images from the tri-modal PET/SPECT/CT experiment were analysed. Additionally, gamma counter was evaluated as a tool for measuring biodistribution of the radiolabelled probes ex vivo.18F-Fluoro-L-Thymidine (18F-FLT) was used in the investigation of the treatment response in the mouse models of pancreatic ductal adenocarcinoma (PDAC). Results from the two studies using mTOR and TGFβ inhibitors are reported. The mTOR inhibitor, rapamycin used 18F-FLT in the PET imaging to study, which aimed to compare the effects of the treatment on proliferation in two mouse models recapitulating the features of human PDAC, namely the KC Pten and KPC. TGFβ inhibitor study characterised the acute impact the administered TGFβ antibody has on proliferation in KPC mice in addition and as opposed to gemcitabine monotherapy, which is currently considered a golden standard in the treatment of pancreatic cancer.This study utilized gamma counting, autoradiography and Ki67 immunohistochemistry (IHC).