Investigations of a Microbial Model for the Determination of Leakage of Root Canal Fillings

Medicine, Dentistry

Chailertvanitkul, Pattama

University:University of Glasgow

关键词: Medicine, Dentistry;   

Others  :  http://theses.gla.ac.uk/74754/1/11007673.pdf

来源: University of Glasgow

【 摘 要 】

This study was a laboratory based investigation of a microbial model for the determination of leakage of root canal fillings using facultative anaerobes, obligate anaerobes, mixed micro-organisms and lipopolysaccharide as markers. Five hundred extracted human teeth with straight, single root canals were prepared chemo-mechanically and obturated with gutta-percha using different techniques and root canal sealers. These teeth were divided randomly into three groups for three major studies; the effect of smear layer on leakage, leakage immediately after obturation and also after long-term storage. A microbial model was developed. The cut end of a tube was sealed around the coronal part of each root canal so that the markers placed therein could move only through the obturated canal space. Each root was placed in a glass bottle containing sterile media and aliquots of the markers were placed in each coronal chamber. The model system was stored in the incubator at 37