Retroactivity to the Output of Transcription Devices: Quantification and Insulation.

Synthetic Biology;Control Systems;Singular Perturbation;Systems Biology;Biomedical Engineering;Electrical Engineering;Biological Chemistry;Microbiology and Immunology;Molecular;Cellular and Developmental Biology;Engineering;Science;Electrical Engineering: Systems

Jayanthi, ShridharGrizzle, Jessy W. ;

University of Michigan

关键词: Synthetic Biology;    Control Systems;    Singular Perturbation;    Systems Biology;    Biomedical Engineering;    Electrical Engineering;    Biological Chemistry;    Microbiology and Immunology;    Molecular;    Cellular and Developmental Biology;    Engineering;    Science;    Electrical Engineering: Systems;   

Others  :  https://deepblue.lib.umich.edu/bitstream/handle/2027.42/95939/jayanthi_1.pdf?sequence=1&isAllowed=y

瑞士|英语

来源: The Illinois Digital Environment for Access to Learning and Scholarship

【 摘 要 】

Traditional engineering often relies on hierarchical design techniques to build com-plex systems from simpler subsystems. This technique requires modularity, a prop-erty that states that the input/output characteristics of a system are not affected byinterconnections. In this work we investigate retroactivity, an impedance-like effectin biomolecular systems that makes the behavior of a system change upon intercon-nection. We show, through analysis and experiments, that retroactivity in syntheticbiology circuits is responsible for substantial changes in a system dynamic response.In order to construct circuits modularly, we propose the design of insulation de-vices, which, similar to insulating amplifiers in electronics, attenuate retroactivityeffects and recover modular behavior. Our technique is based on a novel disturbanceattenuation approach based on singular perturbation theory.