Quantifying Cardiac Microstructure Using Automated Two-Photon Microscopy

Two-Photon Microscopy;Murine Cardiac Structure;Automated Imaging;Fiber Inclination Angle;Biomedical Engineering

Shah, AagamWinslow, Raimond L. ;

Johns Hopkins University

关键词: Two-Photon Microscopy;    Murine Cardiac Structure;    Automated Imaging;    Fiber Inclination Angle;    Biomedical Engineering;   

Others  :  https://jscholarship.library.jhu.edu/bitstream/handle/1774.2/39242/SHAH-THESIS-2014.pdf?sequence=1&isAllowed=y

瑞士|英语

来源: JOHNS HOPKINS DSpace Repository

【 摘 要 】

Two-photon fluorescence microscopy is a three-dimensional imaging technology based on the nonlinear excitation of fluorophores. It has been widely used to map the 3D pattern of neuronal connections in the brain, and for reconstructing anatomical structure of organs such as skin, kidney and others. Here, we present the design of an automated imaging system using low cost, highly modular Automated Volume Imaging Machine (AVIM) and its interface with a Zeiss 7MP microscope. We apply this system to mapping murine cardiac structure.Primary Reader: Dr. Raimond Winslow (Advisor) ([e-mail])Secondary Reader: Dr. Feilim Mac Gabhann, Dr. Sonia Cortassa