The influence of fatty acids in vitro on mammalian cells from species differing in their fatty acyl desaturase capabilities.

["Fatty Acids."]

Giangregorio, Alfredo

University of the Witwatersrand

Others  :  http://wiredspace.wits.ac.za/bitstream/10539/25153/4/Giangregorio%20A_the%20influences%20of%20fatty%20acids%20in%20vi_004.pdf

瑞士|英语

来源: University of the Witwatersrand

【 摘 要 】

Numerous studies have assessed the effects of singlefatty acids on various aspects of lipid metabolism,particularly cancer. Established cell lines have largelybeen used for this purpose. The choice of control cells.however, has often been inappropriate. There is also asurprising lack of knowledge of the effects of fattyacids in the real world, in which normal cells in vivoare presented with mixtures of dietary fatty acids.Before transformed cells can be used as models ofdisease states, it is essential to fully understandfatty acid metabolism in normal (control) cells. Onlythen can experimental findings be extrapolated to theclinical situation with some certainty. This thesis hastherefore, assessed the effects of exogenous fatty acidmixtures on the growth/viability of normal mammaliantissues all cultured under standard conditions, andattempted to elucidate the mechanisms underlying sucheffects.As different mammalian species exhibit different fattyacyl desaturase capabilities, cells from three specieswere chosen, viz. rat, Man and cat, with desaturasecapability decreasing with species, respectively. A widerange of different cell types from each species werestudied due to the known differences in their lipid ( Abbreviation abstract )