Enhancing the efficacy of antimicrobial peptide BM2, against mono-species biofilms, by combining with detergents.

Antimicrobial;peptide;biofilm;Endodontics;detergents

Devi, Arpana Arthi ; Love, Robert ; Monk, Brian

University of Otago

关键词: Antimicrobial;    peptide;    biofilm;    Endodontics;    detergents;   

Others  :  https://ourarchive.otago.ac.nz/bitstream/10523/6919/1/DeviArpanaA2016DClinDent.pdf

美国|英语

来源: Otago University Research Archive

【 摘 要 】

Aim To investigate if a detergent regime could enhance the antimicrobial ability of BM2.Method Strains of Enterococcus faecalis, Streptococcus gordonii, Streptococcus mutans, and Candida albicans were grown from glycerol stocks after confirmation of the strains. After subculturing single colonies were cultured in TSB and CSM liquid media for 24hr to obtain a microbial suspension which was adjusted to OD600nm = 0.5.Dilution series of the peptidomimetic BM2 and detergents were prepared in aqueous solution and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined using a broth micro-dilution method.Further on planktonic cells and monospecies biofilms were exposed to the detergent and BM2 combinations. The efficacy of BM2 and detergents at causing biofilm detachment was measured using a crystal violet based assay.Results Planktonic cells were easier to kill with some of the detergents in isolation or in combination with BM2. SDS and CTAB in combination with BM2 increased the efficacy of BM2 against the test organisms. Tween 20 did not kill any of the test organisms alone or in combination.Biofilms were harder to eradicate and detergent, BM2 combinations gave varied results for the different species tested. Detergents in combination with BM2 did not increase the efficacy of the antimicrobial peptide in disrupting S. mutans biofilm.SDS in combination with BM2 did not increase the efficacy of the antimicrobial peptide in disrupting E. faecalis biofilm at the 24 and 48 hr periods however at the 72 hr period there was disruption of biofilm. CTAB in combination with BM2 did not increase the efficacy of the antimicrobial peptide in disrupting E. faecalis biofilm.iiiCTAB in combination with BM2 increased the efficacy of the antimicrobial peptide in disrupting S. gordonii biofilm at the 24 hr period, however at the 48 and 72 hr no disruption was noted. SDS in combination with BM2 did not increase the efficacy of the antimicrobial peptide in disrupting S. gordonii biofilm.CTAB in combination with BM2 increased the efficacy of the antimicrobial peptide in disrupting C. albicans biofilm at the 24 hr period, however at the 48 and 72 hr no disruption was noted. SDS in combination with BM2 increased the efficacy of the antimicrobial peptide in disrupting C. albicans biofilm at the 24 and 48 hr periods, however at the 72 hr period, the combination did not disrupt C. albicans biofilm.Conclusion Within the limits of this in-vitro study a combination of BM2 and SDS showed antimicrobial action against certain endodontic microorganisms occurring as planktonic cells and in biofilms and offers the potential to develop an effective between visits’ medicament.