Transport connectivity is essential tosustain inclusive growth in developing countries, where manyrural populations and businesses are still considered to beunconnected to the domestic, regional, or global market. TheRural Access Index is among the most important globalindicators for measuring people’s transport accessibility inrural areas where the majority of the poor live. A newmethod to calculate the Rural Access Index was recentlydeveloped using spatial data and techniques. Thecharacteristics of subnational Rural Access Index estimateswere investigated in eight countries: Bangladesh, Ethiopia,Kenya, Mozambique, Nepal, Tanzania, Uganda, and Zambia. Itwas found that for the countries in Africa, road density androad condition are important determinants of the RuralAccess Index. For the South Asian countries, improvement ofroad condition is particularly relevant. The evidencesuggests that significant resources are likely to berequired to achieve universal access through rehabilitatingthe existing road network and expanding the road network.