Assessment of Genotoxic Activity of Petroleum Hydrocarbon-Bioremediated Soil.

Plaza, G. ; Nalecz-Jawecki, G. ; Ulfig, K. ; Brigmon, R. L.

Technical Information Center Oak Ridge Tennessee

关键词: Soil contamination;    Hydrocarbons;    Toxicity;    Remediation;    Petroleum products;   

RP-ID  :  DE2004835096

学科分类：工程和技术（综合）

美国|英语

来源: National Technical Reports Library

【 摘 要 】

The relationship between toxicity and soil contamination must be understood to develop reliable indicators of environmental restoration for bioremediation. Two bacterial rapid bioassays: SOS chromotest and umu-test with and without metabolic activation (S-9 mixture) were used to evaluate genotoxicity of petroleum hydrocarbon-contaminated soil following bioremediation treatment. The soil was taken from an engineered biopile at the Czor Polish oil refinery. The bioremediation process in the biopile lasted 4 years, and the toxicity measurements were done after this treatment. Carcinogens detected in the soil, polyaromatic hydrocarbons (PAHs), were reduced to low concentrations (2 mg/kg dry wt) by the bioremediation process. Genotoxicity was not observed for soils tested with and without metabolic activation by a liver homogenate (S-9 mixture).