Novel Enabling Technologies of Gene Isolation and Plant Transformation for Improved Crop Protection, Summary of the Specific Research and Project Accomplishments.

Cigan, A. ; Torok, T.

Technical Information Center Oak Ridge Tennessee

关键词: Crops;    Genes;    Agricultural products;    Insecticides;    Plants(Botany);   

RP-ID  :  DE141149940

学科分类：工程和技术（综合）

美国|英语

来源: National Technical Reports Library

【 摘 要 】

Meeting the needs of agricultural producers requires the continued development of improved transgenic crop protection products. The completed project focused on developing novel enabling technologies of gene discovery and plant transformation to facilitate the generation of such products. The project had three main components: a. Investigated native chloroplast promoters from different species by testing their ability to drive the expression of a reporter gene in heterologous systems; b. Tested promoter-less vector constructs for the lox-mediated expression of transgenes; c. Screened diverse plant species for the detection of insecticidal, fungicidal, and antiviral activities. To perform the scheduled screening the following main tasks were carried out: scale-up of growing plant material; developed sample preparation protocols for accumulation and storage of plant tissues; grew, accumulated, and freeze-dried plant tissues; optimized subsequent protein extraction protocols; searched for insect, fungal, and viral species that satisfied the screening conditions; developed and performed protocols for antifungal, antiviral, and insecticide tests. All stages planned for the 2-year project were performed under the Work Schedule. The scientific results obtained in the course of experiments represent the basis for further investigations in this field of research.