Prior to the successful flight test validation of a new avionics prototype, participants from Boeing, Honeywell, and United Airlines underwent group training at NASA Langley Research Center. New prototype software for an algorithm which enables greater efficiency in high-density airspace, called Interval Management, was to be incorporated into Electronic Flight Bags and placed in the cockpit for pilot usage. The goals of the training were to teach the flight test pilots how to operate the new software, establish techniques to simultaneously position three aircraft prior to each test scenario, and ensure a common communication protocol among team members when coordinating the position of aircraft for the next scenario. The multi-tiered interactive training regimen consisted of a process that continually built upon previous foundational material. The primary learning elements were 1) a portable computer-based trainer that was provided to the pilots prior to classroom training sessions, 2) classroom learning, 3) full mock-up simulator training, and 4) refresher training just prior to the flight test. Each part of the regimen was designed to repeat and build upon the previous element. The purpose of this Technical Memorandum is to inform the aviation industry how flight training for Interval Management was conducted at Langley Research Center in order to reduce overall development costs of future Interval Management training programs. Secondly, the paper provides insight regarding the decision-making process when attempting to conduct a flight test.