Trends and transitions in the growing season MODerate resolution Imaging Spectroradiometer (MODIS) Normalized Difference Vegetation Index (NDVI) time-series at 250-m resolution were analyzed for the period from 2000 to 2018 to understand recent patterns of vegetation change in ecosystems of the Yukon River basin in interior Alaska. Statistical analysis of changes in the NDVI time series was conducted using the "Breaks for Additive Seasonal and Trend" method (BFAST). This structural change analysis indicated that NDVI breakpoints and negative 18-yr trends in vegetation greenness over the years since 2000 could be explained in large part by the impacts of severe wildfires, commonly affecting shrubland and forested ecosystems at relatively low elevations (< 300 m).