Evaluation of novel methods and materials for seeding tracer particles for particle image velocimetry (PIV) was carried out in the Basic Aerodynamic Research Tunnel (BART) at NASA’s Langley Research Center (LaRC). Seeding of polystyrene latex microspheres (PSLs) from ethanol/water suspensions and from the dry state was carried out using custom built seeders. PIV data generated using the novel methods were found to be in general agreement with data collected using the current seeding methods. Techniques for assessing PSL fouling of wind tunnel surfaces were identified and refined. Initial results suggest that dry seeding PSLs may allow comparable data quality to wet seeding while reducing wind tunnel screen fouling. Results also indicate that further developments to the dry seeding system should focus on increasing single particle flux into the wind tunnel. Modifications to PSLs and seeding equipment to achieve this have been identified and are discussed.